Anti-CD151 [11G5a]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD151 [11G5a]
• Alternate name: CD151 Molecule (Raph Blood Group); Platelet-Endothelial Tetraspan Antigen; CD151 Antigen (Raph Blood Group); Membrane Glycoprotein SFA-1; Tetraspanin-24; CD151 Antigen; Tspan-24; TSPAN24; PETA-3
• Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Tissue-specific biology
• Clone: 11G5a
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: ELISA ; FACS ; IHC ; IF ; IP ; WB
• Description: Clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. CD151 is a cell surface glycoprote involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells.
• Isotype: IgG1
• Myeloma used: P3X63Ag8.653
• Recommended controls: Tonsil/spleen.

Target Details

• Target: CD151 (PETA-3)
• Tissue cell line specificity: Tonsil/spleen.
• Target background: Clone 11G5a recognizes the human CD151 cell surface antigen, also known as PETA-3. CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. CD151 is a cell surface glycoprote involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells.

Applications

• Application: ELISA ; FACS ; IHC ; IF ; IP ; WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Shipping at 4° C

References

• Devbhandari et al. 2011. PLoS One. 6(9):e24901. PMID: 21961047.
• Profiling of the tetraspanin CD151 web and conspiracy of CD151/integrin ?1 complex in the progression of hepatocellular carcinoma.
• Ke et al. 2011. Gastroenterology. 140(5):1629-41.e15. PMID: 21320503.
• CD151 amplifies signaling by integrin a6?1 to PI3K and induces the epithelial-mesenchymal transition in HCC cells.
• Franco et al. 2010. J Biol Chem. 285(50):38756-64. PMID: 20937830.
• Huang et al. 2010. Cancer. 116(23):5440-51. PMID: 20715158.
• The tetraspanin CD151 is required for Met-dependent signaling and tumor cell growth.
• Overexpression of CD151 as an adverse marker for intrahepatic cholangiocarcinoma patients.
• Shi et al. 2010. Hepatology. 52(1):183-96. PMID: 20578262.
• CD151 modulates expression of matrix metalloproteinase 9 and promotes neoangiogenesis and progression of hepatocellular carcinoma.
• Ke et al. 2009. Hepatology. 49(2):491-503. PMID: 19065669.
• Role of overexpression of CD151 and/or c-Met in predicting prognosis of hepatocellular carcinoma.
• Spoden et al. 2008. PLoS One. 3(10):e3313. PMID: 18836553.
• Clathrin- and caveolin-independent entry of human papillomavirus type 16--involvement of tetraspanin-enriched microdomains (TEMs).
• Hasegawa et al. 2007. Lab Invest. 87(9):882-92. PMID: 17632541.
• CD151 dynamics in carcinoma-stroma interaction: integrin expression, adhesion strength and proteolytic activity.
• Zheng et al. 2007. Acta Pharmacol Sin. 28(1):66-72. PMID: 17184584.
• CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation.
• Zheng et al. 2006. Mol Med. 12(9-10):214-20. PMID: 17225869.
• CD151 gene delivery activates PI3K/Akt pathway and promotes neovascularization after myocardial infarction in rats.
• Nishiuchi et al. 2005. Proc Natl Acad Sci U S A. 102(6):1939-44. PMID: 15677332.
• Potentiation of the ligand-binding activity of integrin alpha3beta1 via association with tetraspanin CD151.
• Karamatic Crew et al. 2004. Blood. 104(8):2217-23. PMID: 15265795.
• CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin.
• Yauch et al. 1998. Mol Biol Cell. 9(10):2751-65. PMID: 9763442.
• Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration.