![Immunofluorescence was performed on human brain cell line (U-138MG) using anti-Cathepsin L [4C11] (A) or a commercially sourced antibody (B)](https://cancertools.org/wp-content/uploads/c6327b06-a275-478e-a963-cb0ac531b8b2.png)
Cat. #152642
Anti-Cathepsin L [4C11]
Cat. #: 152642
Sub-type: Primary antibody
Unit size: 100 ug
Target: Cathepsin L, HCL
Class: Monoclonal
Application: IF ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: A*STAR Accelerate Technologies Pte Ltd
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Cathepsin L [4C11]
- Research fields: Biochemistry;Cancer;Cell biology;Cell signaling and signal transduction;Neurobiology
- Clone: 4C11
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IF ; WB
- Description: Cathepsin L is expressed in most cells but in a higher level in cancerous cells. Normal levels of cathepsin L is involved in the regulation of epithelial cell proliferation and differentiation in the skin. It is recognized that many cancer cells secrete cathepsin L to degrade the components of extracellular matrices and basement membranes. This promotes tumor invasion and metastasis. The pro-form of cathepsin L exists as a molecular size of 50kDa. The pro-form could be degraded and secreted out as two smaller forms of cathepsin L. These smaller forms are known as the mature forms. The pro-forms and mature forms are secreted out of the healthy cells. However, there is a significant increase in levels of pro-forms that are secreted out of the cancerous cells. Monoclonal antibodies generated against pro-form of cathepsin L would be useful for diagnostic purposes.
- Immunogen: GST-HCL fusion protein
- Isotype: IgM
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Human brain cell lysates, U-138MG cell line
Target Details
- Target: Cathepsin L, HCL
- Tissue cell line specificity: Human brain cell lysates, U-138MG cell line
- Target background: Cathepsin L is expressed in most cells but in a higher level in cancerous cells. Normal levels of cathepsin L is involved in the regulation of epithelial cell proliferation and differentiation in the skin. It is recognized that many cancer cells secrete cathepsin L to degrade the components of extracellular matrices and basement membranes. This promotes tumor invasion and metastasis. The pro-form of cathepsin L exists as a molecular size of 50kDa. The pro-form could be degraded and secreted out as two smaller forms of cathepsin L. These smaller forms are known as the mature forms. The pro-forms and mature forms are secreted out of the healthy cells. However, there is a significant increase in levels of pro-forms that are secreted out of the cancerous cells. Monoclonal antibodies generated against pro-form of cathepsin L would be useful for diagnostic purposes.
Applications
- Application: IF ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
