Cat. #151073
Anti-APC [Ali 12-28]
Cat. #: 151073
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Adenomatous Polyposis Coli (APC)
Class: Monoclonal
Application: WB ; ELISA ; IHC ; IF ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-APC [Ali 12-28]
- Cancer: Digestive / Gastrointestinal cancer
- Cancers detailed: Colorectal
- Research fields: Cancer;Tissue-specific biology;Cell biology;Cell signaling and signal transduction;Genetics;Neurobiology;Stem cell biology
- Clone: Ali 12-28
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 310 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: WB ; ELISA ; IHC ; IF ; IP ; WB
- Description: Ali 12-28 may be used for the detection of APC expression and APC mutations.
- Immunogen: N-terminal APC-MBP fusion protein
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
- Recommended controls: Cancer cell lines: SW480, LOVO, DLD1, SW837
Target Details
- Target: Adenomatous Polyposis Coli (APC)
- Molecular weight: 310 kDa
- Tissue cell line specificity: Cancer cell lines: SW480, LOVO, DLD1, SW837
- Target background: The adenomatous polyposis coli (APC) tumour suppressor gene is mutated (often by deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and in over 80% of colorectal cancers.
Applications
- Application: WB ; ELISA ; IHC ; IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Hadjihannas et al. 2012. EMBO Rep. 13(4):347-54. PMID: 22322943.
- Cell cycle control of Wnt/?-catenin signalling by conductin/axin2 through CDC20.
- Valvezan et al. 2012. J Biol Chem. 287(6):3823-32. PMID: 22184111.
- Tanneberger et al. 2011. J Biol Chem. 286(22):19204-14. PMID: 21498506.
- Structural and Fn characterization of the Wnt inhibitor APC membrane recruitment 1 (Amer1).
- Arnold et al. 2009. EMBO J. 28(5):500-12. PMID: 19131971.
- The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.
- Efstathiou et al. 1998. Proc Natl Acad Sci U S A. 95(6):3122-7. PMID: 9501226.
- Intestinal trefoil factor controls the expression of the adenomatous polyposis coli-catenin and the E-cadherin-catenin complexes in human colon carcinoma cells.
