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#152711

Anti-5T4 [B3F1]

Cat. #152711

Anti-5T4 [B3F1]

Cat. #: 152711

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-4 weeks

Target: 5T4 oncofetal glycoprotein

Class: Monoclonal

Application: ELISA ; FACS ; IF ; Fn ; WB

Reactivity: Mouse

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Peter Stern

Institute: Cancer Research UK, Manchester Institute

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-5T4 [B3F1]
  • Alternate name: Trophoblast Glycoprotein; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; WAIF1; M6P1; 5T4; 5T4AG
  • Research fields: Cancer;Stem cell biology
  • Clone: B3F1
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 46.5 kDa
  • Strain: C57BL/6
  • Reactivity: Mouse
  • Host: Mouse
  • Application: ELISA ; FACS ; IF ; Fn ; WB
  • Description: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.
  • Immunogen: Q9Z0L0
  • Immunogen uniprot id: Q9Z0L0
  • Isotype: IgG2a

Target Details

  • Target: 5T4 oncofetal glycoprotein
  • Molecular weight: 46.5 kDa
  • Target background: Monoclonal antibody directed against 5T4, with use as a prognostic aid of tumours. Background and Research Application The murine 5T4 monoclonal antibody, clone B3F1, identifies the human 5T4 oncofoetal antigen, also known as trophoblast glycoprotein. 5T4 is a highly glycosylated protein expressed by trophoblast and a few specialized adult epithelia and is an antagonist of the Wnt/ ?-catenin signalling pathway. Up-regulation of 5T4 expression in some cancers is associated with poor clinical outcome; overexpression of human 5T4 cDNA in epithelial cells can alter their morphology and motility, supporting a role for such functions in cancer and development. 5T4 has limited expression in normal tissues, but is widespread in tumours, thus its expression is used as a prognostic aid. 5T4 is typically found in colorectal, ovarian and gastric tumours. 5T4 is a potential target for T cells, in antibody-directed immunotherapy.

Applications

  • Application: ELISA ; FACS ; IF ; Fn ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: Anti-5T4 [B1C3] ; Anti-5T4 [B5C9] ; Anti-5T4 [B5C12]

References

  • Castro et al. 2012. Cancer Immunol Immunother. 61(7):1005-18. PMID: 22127365.
  • Southgate et al. 2010. PLoS One. 5(4):e9982. PMID: 20376365.
  • CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells.
  • Ward et al. 2003. J Cell Sci. 116(Pt 22):4533-42. PMID: 14576347.
  • The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency.
  • Woods et al. 2002. Biochem J. 366(Pt 1):353-65. PMID: 12003637.
  • Characterization of the murine 5T4 oncofoetal antigen: a target for immunotherapy in cancer.

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