#152733

Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb

Cat. #152733

Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb

Cat. #: 152733

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 3-5 days

Target: 5-Hydroxymethylcytosine (5hmC)

Class: Monoclonal

Application: IF ; IP ; DB

Reactivity: All

Host: Rat

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Wolf Reik

Institute: Babraham Institute

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb
  • Research fields: Genetics;Stem cell biology
  • Clone: AB3/63.3
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Reactivity: All
  • Host: Rat
  • Application: IF ; IP ; DB
  • Description: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
  • Immunogen: 5-Hydroxymethylcytosine conjugated to BSA
  • Isotype: IgG2a kappa
  • Recommended controls: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA

Target Details

  • Target: 5-Hydroxymethylcytosine (5hmC)
  • Tissue cell line specificity: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
  • Target background: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.

Applications

  • Application: IF ; IP ; DB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -20° C
  • Shipping conditions: Shipping at 4° C

References

  • Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.
  • Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.
  • Alterations of epigenetic signatures in hepatocyte nuclear factor 4a deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.
  • Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis.
  • Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.
  • Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
  • Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
  • 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.