Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-5-Hydroxymethylcytosine (5hmc) [AB3/63.3] mAb
• Research fields: Genetics;Stem cell biology
• Clone: AB3/63.3
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: All
• Host: Rat
• Application: IF ; IP ; DB
• Description: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.
• Immunogen: 5-Hydroxymethylcytosine conjugated to BSA
• Isotype: IgG2a kappa
• Recommended controls: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA

Target Details

• Target: 5-Hydroxymethylcytosine (5hmC)
• Tissue cell line specificity: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA
• Target background: The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base. It is formed from the DNA base cytosine by adding a methyl group and then a hydroxy group. Every mammalian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; generally, the levels of 5-hydroxymethylcytosine increase with age. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethylcytosine may regulate gene expression or initiate DNA demethylation.

Applications

• Application: IF ; IP ; DB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -20° C
• Shipping conditions: Dry ice

References

• Zhang et al. 2014. PLoS One. 9(1):e84925. PMID: 24427299.
• Cao et al. 2014. Theriogenology. 81(3):496-508. PMID: 24315686.
• Alterations of epigenetic signatures in hepatocyte nuclear factor 4a deficient mouse liver determined by improved ChIP-qPCR and (h)MeDIP-qPCR assays.
• Dynamic reprogramming of 5-hydroxymethylcytosine during early porcine embryogenesis.
• Ficz et al. 2011. Nature. 473(7347):398-402. PMID: 21460836.
• Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
• Wossidlo et al. 2011. Nat Commun. 2:241. PMID: 21407207.
• 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming.