BRIN-BG7 cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: BRIN-BG7 cell line
• Research fields: Disease (not cancer)
• Tool sub type: Hybrid cell line
• Parental cell: RINm5F cell line
• Organism: Rat
• Tissue: Pancreas
• Disease: Diabetes
• Morphology: Epitheloid
• Growth properties: Adherent
• Model: Transgenic
• Model description: Hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma)
• Crispr: No
• Description: A hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic beta cell function as they showed insulin output 5–10 times greater than parent RINm5F cells
• Application: producing pure insulin secreting cells when stimulated

Applications

• Application: producing pure insulin secreting cells when stimulated

Handling

• Format: Frozen
• Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
• Temperature: 37° C
• Atmosphere: 5% CO2
• Shipping conditions: Dry Ice
• Storage conditions: Liquid Nitrogen
• Initial handling information: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cmÂ? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cmÂ? can be expected.
• Mycoplasma free: Yes

References

• McClenaghan NH et al. 1999. J Mol Med. 77(1):235-43 PMID: 9930971.