Cat. #161630
BRIN-BG5 cell line
Cat. #: 161630
Sub-type: Hybrid cell line
Organism: Rat
Tissue: Pancreas
Disease: Diabetes
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Peter Flatt and Neville McClenaghan
Institute: Ulster University
Primary Citation: McClenaghan NH et al. 1996. J Endocrinol.;148(3):409-17 PMID: 8778219
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BRIN-BG5 cell line
- Research fields: Disease (not cancer)
- Tool sub type: Hybrid cell line
- Parental cell: RINm5F cell line
- Organism: Rat
- Tissue: Pancreas
- Disease: Diabetes
- Morphology: Epitheloid
- Growth properties: Adherent
- Model: Transgenic
- Model description: Hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma)
- Crispr: No
- Description: A hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic beta cell function as they showed insulin output 5â10 times greater than parent RINm5F cells
- Application: producing pure insulin secreting cells when stimulated
Applications
- Application: producing pure insulin secreting cells when stimulated
Handling
- Format: Frozen
- Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
- Temperature: 37° C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Initial handling information: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 28hrs. At confluence 105 cells/cm� can be expected.
- Mycoplasma free: Yes
References
- McClenaghan NH etal. 1999. J Mol Med. 77(1):235-43 PMID: 9930971.
