BRIN-BG5 cell line

cell line represents an important stable glucose-responsive insulinsecreting ?-cell line for future studies of pancreatic beta cell function

Contributors

Inventor Institute
Peter Flatt and Neville McClenaghan Ulster University
Cat. #: 161630
Tool sub type: Hybrid cell line
Research Fields: Disease (not cancer)
Organism: Rat
Tissue: Pancreas
Model: Transgenic
Morphology: Epitheloid
Growth properties: Adherent
Primary citation: McClenaghan NH et al. 1996. J Endocrinol.;148(3):409-17 PMID: 8778219
Product description: A hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic beta cell function as they showed insulin output 5–10 times greater than parent RINm5F cells
CRISPR: No
Parental cell line: RINm5F cell line
Model description: Hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma)
disease: Diabetes
Initial handling information: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 28hrs. At confluence 105 cells/cm� can be expected.
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

McClenaghan NH etal. 1999. J Mol Med. 77(1):235-43 PMID: 9930971.

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