MDA-MB-231 TetOn-3G ROCK1 cell line

Useful for the study of breast cancer cell cytoskeleton reorganisation and cell migration. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.

Contributors

Inventor Institute
Heather Mckinnon ; Justin Bower ; Daniel Croft Cancer Research UK, Glasgow: The Beatson Institute
Cat. #: 154150
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Cell biology;Drug development;Genetics
Organism: Human
Tissue: Breast
Model: Transgenic
Growth properties: Adherent cell line. Membrane blebbing/cytoskeleton reorganisation and migration induced in the presence of doxycycline.
Cancer Types In Detail: Triple negative breast (TNBC)
Alternate name: Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase I, ROCK-I, p16 ROCK-1, p16ROCK
Product description: This cell line has been engineered to enable tetracycline inducible expression of the kinase ROCK1. ROCK1 is a serine/threonine kinase which acts as a key regulator of the actin-myosin cytoskeleton and cell polarity. ROCK1 promotes contractile force generation and therefore is a modulator of cell motility and tumour cell invasion. The cell line is a derivative of a human breast cancer cell line shown to reliably metastasize to clinically relevant sites (the lungs and lymph nodes) when implanted orthotopically in mice (i.e. when implanted in the breast pad of mice). The cell line expresses luciferase enabling detection of the location of the cells using bioluminescent imaging. Useful for the study of breast cancer cell cytoskeleton reorganisation and cell migration. This cell line is derived from a triple negative breast cancer (TNBC) meaning it does not express oestrogen, progesterone or HER2 receptors.
Conditional: Yes
Conditional description: MDA-MB-231 D3H2LN-Luc cell line that expresses tetracycline (Tet)-regulated transactivator Tet-On 3G regulated expression of ROCK1. Therefore ROCK1 expression is induced in the presence of doxycycline.
Additional notes: Tet-On System owned by TET Systems GmbH & Co. KG.
Parental cell line: MDA-MB-231 D3H2LN-Luc cell line
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: 10% FBS/MEM-EBSS/NEAA/NaPyr/Glutamine
Recommended controls: MDA-MB-231 D3H2LN-Luc parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Unbekandt et al. 2014. Cell Commun Signal. 12:54. PMID: 25288205.

A novel small-molecule MRCK inhibitor blocks cancer cell invasion.

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