#153793

pTBNde Vector

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Cat. #153793

pTBNde Vector

Cat. #: 153793

Sub-type: pBluescript KS

Availability: Please enquire for quantities and pricing

Bacterial Resistance: Ampicillin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Prof Emanuele Buratti

Institute: International Centre For Genetic Engineering And Biotechnology (ICGEB)

Tool Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pTBNde Vector
  • Research fields: Genetics
  • Tool sub type: pBluescript KS
  • Backbone size without insert: 2958
  • Bacterial resistance: Ampicillin
  • Description: Concentration 1.35mg/ml
  • Additional notes: This vector is a typical hybrid minigene that contains a ‘‘simplified’’ version of the gene that will be evaluated for pre-mRNA splicing. In particular, it contains at the 5' end an alfa-globin gene promoter and SV40 enhancer sequences to allow polymerase II transcription in the transfected cell lines. This is followed by a series of exonic and intronic sequences that may derive from a reporter gene or from the gene context itself. In this case, the reporter gene is composed of a-globin and fibronectin exons, while at the 3' end a functionally competent polyadenylation site, derived from the alfa-globin gene, is present. The genomic DNA region of interest that contains a putative splicing mutation is introduced into the minigene in a unique restriction site (NdeI).

Application Details

  • Application notes: Concentration 1.35mg/ml

References

  • Pagani et al. 2000. J Biol Chem. 275(28):21041-7. PMID: 10766763.
  • Splicing factors induce cystic fibrosis transmembrane regulator exon 9 skipping through a nonevolutionary conserved intronic element.