Cat. #160576
pROSA26-GNrep vector
Cat. #: 160576
Sub-type: pROSA26
Availability: Please enquire for quantities and pricing
Bacterial Resistance: Neomycin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Dr Claudio Franco
Institute: Instituto de Medicina Molecular JoĂÂÄšÂo Lobo Antunes
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: pROSA26-GNrep vector
- Research fields: Developmental biology
- Tool sub type: pROSA26
- Backbone size without insert: 1424bp
- Bacterial resistance: Neomycin
- Description: This vector was used to generate a reporter mouse for front-rear cell polarity (mouse linked above in the "Related" tab).
- Additional notes: As a backbone for this vector, pROSA26�DV2 vector that carries Rosa26 5′� and 3′�homology arms and enables rapid, modular, and efficient generation of plasmid DNA suitable for homologous recombination into the Rosa26 genomic locus of mouse embryonic stem cells (mESCs) was used (Nyabi et al., 2009, PMID:19279185). This plasmid allows for multisite Gateway recombination�mediated insertion of three entry DNA sequences which was used here to generate pROSA26�GNrep vector, containing a CAGGS synthetic promoter followed by a loxP�dSTOP�loxP cassette, the GNrep cassette, by a selection cassette pA�FRT�PGK�Neo�pA�FRT (linked in "Related" above).
Application Details
- Application notes: This vector was used to generate a reporter mouse for front-rear cell polarity (mouse linked above in the "Related" tab).
Related Tools
- Related tools: GNrep mouse
References
- Barbacena et al. 2019. Genesis. 57(6):e23299. PMID: 30990965.
