#161592

pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 472) fusion protein

Cat. #161592

pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 472) fusion protein

Cat. #: 161592

Sub-type: pCR-Blunt II-TOPO™ cloning vector

Bacterial Resistance: Kanamycin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Gontzal GarcĂ­a del CaĂąo

Institute: University of the Basque Country

Primary Citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool Details
Handling
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 472) fusion protein
  • Tool sub type: pCR-Blunt II-TOPO™ cloning vector
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Insert: CB1414–472
  • Total vector size: 3710 bp
  • Backbone size without insert: 3519 bp
  • Insert size: 191 bp
  • Insert species: Human
  • Promoter: Not applicable
  • Bacterial resistance: Kanamycin
  • Description: Purified plasmid with a DNA insert spanning codons corresponding to amino acids CB1414-472 of the cytosolic tail of the human CB1 receptor (CB1414–472) with BamHI and NotI restriction sites for subcloning
  • Application: Subcloning
  • Cloning information: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Additional notes: Contact the inventor for further information.

Handling

  • Storage conditions: Refrigerated at 4 oC (or frozen at -20 oC for long periods)
  • Storage buffer: Double distilled water
  • Shipping conditions: At room temperature in water solution

Application Details

  • Application: Subcloning
  • Application notes: Subcloning

References

  • GĂłmez-Caballero et al. (2021). Mikrochim Acta, 188:368. doi:10.1007/s00604-021-05029-z