#161593

pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 442) fusion protein

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Cat. #161593

pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 442) fusion protein

Cat. #: 161593

Sub-type: pCR-Blunt II-TOPO™ cloning vector

Bacterial Resistance: Kanamycin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Gontzal García del Caño

Institute: University of the Basque Country

Primary Citation: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool Details
Handling
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pCR-Blunt II-TOPO™ cloning vector for GST and human CB1 receptor (AA 414 to 442) fusion protein
  • Research fields: Biochemistry;Cell biology;Molecular Biology;Neurobiology
  • Tool sub type: pCR-Blunt II-TOPO™ cloning vector
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Insert: CB1414–442
  • Total vector size: 3620 bp
  • Backbone size without insert: 3520 bp
  • Insert size: 101 bp
  • Insert species: Human
  • Promoter: Not applicable
  • Bacterial resistance: Kanamycin
  • Description: Purified plasmid with a DNA insert spanning codons corresponding to amino acids CB1414-442 of the cytosolic tail of the human CB1 receptor (CB1414–442) with BamHI and NotI restriction sites for subcloning
  • Application: Subcloning
  • Cloning information: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Additional notes: Contact the inventor for further information.

Handling

  • Storage conditions: Refrigerated at 4 oC (or frozen at -20 oC for long periods)
  • Storage buffer: Double distilled water
  • Shipping conditions: At room temperature in water solution

Application Details

  • Application: Subcloning
  • Application notes: Subcloning

References

  • Saumell-Esnaola et al. 2022 Microb Cell Fact.21(1):192. PMID: 36109736
  • Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.