Fungi Endoplasmic Reticulum Tracker 5 small molecule (tool compound)

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: Fungi Endoplasmic Reticulum Tracker 5 small molecule (tool compound)
• Research fields: Cell biology
• Molecular formula: C25H25BF4N6O2Na
• Tool sub type: Fluorescent Probe
• Description: In fungal cells, the endoplasmic reticulum (ER) harbours several of the enzymes involved in the biosynthesis of ergosterol, an essential membrane component, making this organelle the site of action of antifungal azole drugs, used as a first-line treatment for fungal infections. This marker provides specific fluorescent labelling of this organelle in cells of live pathogenic fungi. Specifically, the marker is an antifungal azole-based fluorescent tracking reagent of the ER in live fungal cells...
• Application: Tracking of the ER in live fungal cells (e.g. Candida).
• Selectivity: Highly specific for Endoplasmic Reticulum.
• Molecular weight: 550.2014
• Solubility: DMSO
• Additional notes: This tracker is optimised for use in fungi, not mammalian or plant cells and has shown superiority to other commercially available ER trackers. It has been evaluated in a panel of Candida, including C. albicans and C. glabrata which are considered the two most common fungal pathogens relevant to human infection. There is weak background signal and high specificity. PMID: 30427174

Handling

• Storage conditions: -15° C to -25° C, light protection

Application Details

• Application: Tracking of the ER in live fungal cells (e.g. Candida).

References

• Benhamou et al. 2018. ACS Chem Biol. 13(12):3325-3332. PMID: 30427174.