RPAN001 Pancreatic cancer organoid

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: RPAN001 Pancreatic cancer organoid
• Alternate name: RPAN001, RPAN1
• Cancer: Adenocarcinoma;Pancreatic cancer
• Cancers detailed: Adenocarcinoma;Pancreatic cancer
• Age y o : 66
• Research fields: Cancer
• Tool sub type: Organoids
• Organism: Human
• Tissue: Pancreas
• Gender: Female
• Growth properties: Suspension
• Application: 3D cell culture, High-throughput screening
• Description: A series of novel patient-derived organoids (PDOs) have been constructed from different tumor tissue types under the Fukushima Translational Research Project, designated as F-PDO. F-PDOs form large cell clusters with a morphology similar to the original tumor and can be cultured for more than six months. Our comparative histological and comprehensive gene expression analyses have shown that the characteristics of F-PDOs were similar to their source tumors, even after long-term growth in culture conditions
• Biosafety level: 2

Handling

• Format: Frozen
• Passage number: 5
• Growth medium: Cancer Cell Expansion Media plus Fujifilm Wako Pure Chemical, Ltd.) with 10 ng/mL bFGF, 10 ĞM Y-27632, 1 ĞM A-83-01, and 100 ng/mL R-Spondin-1.
• Temperature: 37° C
• Atmosphere: 5% CO2
• Volume: 1 ml
• Storage medium: CELLBANKER 2
• Storage conditions: Liquid Nitrogen
• Shipping conditions: Dry ice
• Characterisation tests: Pathogen and stelility test; Comprehensive gene expression analysis; Cancer panel sequencing; Whole exome sequencing; Anticancer drug susceptibility testing
• Mycoplasma free: Yes
• Biosafety level: 2

Application Details

• Application: 3D cell culture, High-throughput screening

Documentation

• User manual
• Brochure