#153341

RalA Floxed Mouse

Cat. #153341

RalA Floxed Mouse

Cat. #: 153341

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Tumorigenesis

Model: Transgenic

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Contributor

Inventor: Chris Marshall

Institute: The Institute of Cancer Research

Tool Details
Handling
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: RalA Floxed Mouse
  • Alternate name: RAS like proto-oncogene A, RALA, Ras-related protein Ral-A, RAL
  • Research fields: Cancer;Cell signaling and signal transduction;Developmental biology;Genetics;Neurobiology
  • Tool sub type: Mouse
  • Disease: Tumorigenesis
  • Model: Transgenic
  • Conditional description: Requires expression of Cre recombinase to mediate excision of the lox P sites generating a non-functional Ral A protein
  • Description: Ras is a family of proteins which exist in all animal cell lineages and organs. The Ras protein family belongs to a group of proteins called small GTPases which are involved in cellular signal transduction. Ras activation, by incoming cellular signals, switches on other proteins which causes downstream activation of genes involved in cell growth, differentiation and survival. Mutations in Ras genes can lead to the production of permanently activated Ras proteins, overactive signalling inside the cell, even in the absence of incoming signals resulting in uncontrolled cell growth and division which can ultimately lead to cancer. RAL small GTPases, encoded by the Rala and Ralb genes, are members of the Ras superfamily and can act as downstream effectors of Ras. Although both proteins are highly similar, distinct functions have been identified. Ras-related protein Ral-A protein is encoded on chromosome 7, and has been implicated in insulin secretion, epithelial cell polarity neurite branching, neuronal polarity and GLUT4 translocation. Ras-related protein Ral-B protein RALB in migration/invasion, tumor cell survival, autophagy and TBK1 activation.
  • Genetic background: Lox P sequences were inserted to flank exons 2 and 3 of the Ral A gene
  • Phenotype: Tumorigenesis
  • Zygosity: Heterozygous
  • Strain: C57BL/6
  • Production details: Lox P sequences were inserted to flank exons 2 and 3 of the Ral A gene
  • Breeding information: Animals are healthy, fertile and have a normal life span

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Ras-related protein Ral-A

Related Tools

  • Related tools: RalB Floxed Mouse

References

  • Peschard et al. 2012. Curr Biol. 22(21):2063-8. PMID: 23063435.
  • Genetic deletion of RALA and RALB small GTPases reveals redundant functions in development and tumorigenesis.