Cat. #151476
Raf-/- KO Mouse
Cat. #: 151476
Sub-type: Mouse
Availability: 8-10 weeks
Disease: Cancer
Model: Knock-Out
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Catrin Pritchard
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: Raf-/- KO Mouse
- Research fields: Cancer;Cell signaling and signal transduction;Genetics;Neurobiology
- Tool sub type: Mouse
- Disease: Cancer
- Model: Knock-Out
- Conditional: No
- Description: The Raf-/- embryo phenotype shows defects in vascularisation and placental development as well as increased apoptosis of many tissues.
- Genetic background: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
- Phenotype: Homozygous Raf-1-/- mice die in embryogenesis.
- Zygosity: Heterozygous
- Production details: Raf-1 targeting vectors, where exons 10-13 were replaced with a resistance marker, were transfected into 129Ola ES cells. Successfully targeted ES cells containing homologous recombination events were selected and injected into blastocysts, Chimeric offspring were mated to MF-1 outbred background and the the C57BL6 inbred strain.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Raf1+/- heterozygous knockout
Related Tools
- Related tools: MEF RAF1 KO Cell Line
References
- Hser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
- MEK kinase activity is not necessary for Raf-1 function.
