MDR KO Mouse

Deletion of the MDR in this model organism recapitulates the full spectrum of CLL-associated lymphoproliferations in humans.

Contributors

Inventor Institute
Riccardo Dalla-Favera The Trustees of Columbia University in the City of New York
Cat. #: 154094
Research Fields: Cancer;Immunology
Target: Minimal deleted region of h13q14/m14qC3
disease: Chronic Lymphoblastic Leukemia
Model: Knock-Out
Zygosity: Homozygous
Strain: C57BL/6
Product description: Deletion of human chromosomal region 13q14 (mouse 14qC3) represents the most common genetic aberration in B-cell chronic lymphocytic leukaemia (CLL), a neoplasm of mature B lymphocytes. 13q14 deletions are commonly large and heterogeneous in size and affect multiple genes. Contained within the 13q14 region is the 0.11 megabase-long MDR, which encompasses the DLEU2 gene and miR-15a/16-1 cluster. Deletion of the MDR in this model organism recapitulates the full spectrum of CLL-associated lymphoproliferations in humans.
Conditional: No
Genetic background: A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele
Production details: A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele
Additional notes: Knockout mouse of the MDR chromosomal region in m14qC3/h13q14, which encodes the DLEU2 gene and the miR15a/16-1 microRNA cluster
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Klein et al. 2010. Cancer Cell. 17(1):28-40. PMID: 20060366.

The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.

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