#154094

MDR KO Mouse

Cat. #154094

MDR KO Mouse

Cat. #: 154094

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Chronic Lymphoblastic Leukemia

Model: Knock-Out

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Riccardo Dalla-Favera

Institute: The Trustees of Columbia University in the City of New York

Tool Details
Handling
Target Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: MDR KO Mouse
  • Research fields: Cancer;Immunology
  • Tool sub type: Mouse
  • Disease: Chronic Lymphoblastic Leukemia
  • Model: Knock-Out
  • Model description: Knockout mouse of the MDR chromosomal region in m14qC3/h13q14, which encodes the DLEU2 gene and the miR15a/16-1 microRNA cluster
  • Conditional: No
  • Description: Deletion of human chromosomal region 13q14 (mouse 14qC3) represents the most common genetic aberration in B-cell chronic lymphocytic leukaemia (CLL), a neoplasm of mature B lymphocytes. 13q14 deletions are commonly large and heterogeneous in size and affect multiple genes. Contained within the 13q14 region is the 0.11 megabase-long MDR, which encompasses the DLEU2 gene and miR-15a/16-1 cluster. Deletion of the MDR in this model organism recapitulates the full spectrum of CLL-associated lymphoproliferations in humans.
  • Genetic background: A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele
  • Zygosity: Homozygous
  • Strain: C57BL/6
  • Production details: A targeting vector was devised in order to flank MDR with frt-sites. DNA fragments of the 129/Sv-14qC3 were inserted into the targeting vector. Chimeras were obtained from correctly targeted ES cell colonies after injection of the targeted W9.5 ES clones (129/SvEvTac) into blastocysts derived from C57BL/6 mice, and gave rise to MDRfl/+ mice. The chimeras were then crossed with 129/SvCAGGS-Flpe to generate a MDR null allele
  • Additional notes: Knockout mouse of the MDR chromosomal region in m14qC3/h13q14, which encodes the DLEU2 gene and the miR15a/16-1 microRNA cluster

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Minimal deleted region of h13q14/m14qC3

References

  • Klein et al. 2010. Cancer Cell. 17(1):28-40. PMID: 20060366.
  • The DLEU2/miR-15a/16-1 cluster controls B cell proliferation and its deletion leads to chronic lymphocytic leukemia.