Cat. #161902
TOV-3121EP
Cat. #: 161902
Organism: Human
Tissue: Ovary
Disease: Cancer
£575.00
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Contributor
Inventor: Anne-Marie Mes-Masson and Diane Provencher
Institute: Centre Hospitalier de L’université de Montréal
Primary Citation: Sauriol, et al. 2020. Cancers(Basel). 8,12(8):2222. PMID: 32784519
Tool Details
*FOR RESEARCH USE ONLY
- Name: TOV-3121EP
- Organism: Human
- Tissue: Ovary
- Donor: Donor was 62 years at age of diagnosis with stage 3 IIIC fallopian tube high-grade serous adnocarcinoma and a TP53 mutation
- Disease: Cancer
- Morphology: Formed spheroids with small, compact cores surrounded by loosely aggregated cells with an irregular margin
- Growth properties: Mixed
- Crispr: No
- Description: Cell line derived from solid tumor of patient with Stage 3 IIC fallopian tube-HGSC and had received neo-adjuvant chemotherapy of carboplatin/taxol prior to specimen collection in 2006. Cell line had intermediate sensitivity to carboplatin and did not form SC or IP tumors in NRG mice.
- Production details: Tissue was scraped into 100mm plate with complete OSE medium and maintained for 40 days with weekly culture medium replacement
- Biosafety level: 1
Handling
- Format: Frozen
- Growth medium: OSE medium, 10% FBS, 0.5ug/ml apmphotericin B and 50ug/ml gentamycin sulfate
- Temperature: 37° C
- Atmosphere: 7% O2 and 5% CO2
- Shipping conditions: Dry Ice
- Storage conditions: Liquid Nitrogen
- Cultured in antibiotics: 2.5ug/ml amphotericin B and 50 ug/ml gentamicin
- Characterisation tests: Whole exome sequencing, Gene expression microarray, IHC, Western Blot, etc.
- Str profiling: TH01: 9.3 / D21S11: 30,32.2 / D5S818: 10,13 / D13S317: 12 / D7S820: 10 / D16S539: 12 / CSF1PO: 10,12 / vWA: 16,18 / TPOX: 8 / AMEL: X
References
- Meng, et al. 2023. J Anim Sci. 3, 101:skad215. PMID: 37351870
