Cat. #151891
T47D-182R-1 Cell Line
Cat. #: 151891
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Anne Lykkesfeldt
Institute: Danish Cancer Society
Primary Citation: Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: T47D-182R-1 Cell Line
- Cancer: Breast cancer
- Cancers detailed: Breast carcinoma;Fulvestrant resistant
- Research fields: Cancer;Drug development
- Tool sub type: Continuous
- Parental cell: T47D
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Model: Tumour line
- Description: The T47D-182R-1 cell line is an adherent breast cancer cell line resistant to fulvestrant (Faslodex). It is derived from the human breast cancer cell line - T47D/S5 by long term treatment with 100 nM fulvestrant. T47D-182R-1 is adherent and the morphology is epithelial. Resistance will inevitably occur for the second-line therapy fulvestrant. Therefore, this is a highly valuable tool in extending knowledge of acquired therapeutic resistance to ultimately find targeted treatments to resistant tumour cells. As well as treatment that can inhibit or delay the emergence of resistance.
- Application: Determining molecular mechanisms around fulvestrant resistance
- Production details: Human breast cancer cell line derived from T47D/S5 by long term treatment with 100 nM fulvestrant. Grow with 5% fetal bovine serum and 100 nM fulvestrant. Oestrogen and progesterone receptor negative.Passage 166 (AL3372, AL3381)
- Cellosaurus id: CVCL_1D34
Target Details
- Target: Oestrogen receptor
Applications
- Application: Determining molecular mechanisms around fulvestrant resistance
- Application notes: Oestrogen and progesterone receptor negative.
Handling
- Format: Frozen
- Passage number: Passage 166 (AL3372, AL3381)
- Growth medium: Phenol red free RPMI 1640 + 5% FCS + glutamax + 8ug Insulin/ml + 100 nM fulvestrant. Grow with 5% fetal bovine serum and 100 nM fulvestrant.
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: T47D/S2 Cell Line
References
- Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
- Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
- Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
