RPE1 PBRM1 KO2 + PBRM1

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: RPE1 PBRM1 KO2 + PBRM1
• Parental cell: RPE1-hTERT
• Clone: 2 (also called B16)
• Organism: Human
• Gender: Female
• Tissue: retinal epithelium
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Genetically modified; immortalised non-cancer cells
• Model description: Conditional re-expression of wild type PBRM1 gene
• Crispr: Yes
• Conditional: Yes
• Conditional description: Conditional expression of wild type PBRM1 gene following deoxycycline treatment
• Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
• Description: RPE1-hTERT cell line knockout for PBRM1 (clone 2, also called B16), stably expressing DOX-inducible PBRM1 construct. This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
• Application: Chromatin remodelling biology studies
• Biosafety level: 1
• Cellosaurus id: CVCL_4388

Applications

• Application: Chromatin remodelling biology studies

Handling

• Format: Frozen
• Growth medium: DMEM /Ham F12 nutrient mixture + 10S + 1% Penicillin/Streptomycin
• Temperature: 37C
• Atmosphere: 5% CO2 in air
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Cultured in antibiotics: 1% Penicillin/Streptomycin
• Str profiling: See Cellosaurus profile of parental cell line

References

• Unpublished