#161759

OvsahoCarbo cell line

Cat. #161759

OvsahoCarbo cell line

Cat. #: 161759

Organism: Mouse

Tissue: Ovary

Model: Mutant

£575.00

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Contributor

Inventor: Michelle Lockley

Institute: Queen Mary University of London

Primary Citation: J I Hoare et al. 2022. Gynecol Oncol. 167(1):96-106. PMID: 35918200

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: OvsahoCarbo cell line
  • Parental cell: Ovsaho cell line
  • Organism: Mouse
  • Gender: Female
  • Tissue: Ovary
  • Growth properties: Adherent
  • Model: Mutant
  • Model description: Platinum-resistant HGSC in vivo cell line
  • Crispr: No
  • Description: Cells show increased expression of the ABCB1 drug efflux pump as well as reversion mutations in BRCA genes and loss of BRCA promoter-methylation. Platinum-resistant HGSC cancer cells with a growth advantage in the presence of therapy that expand to create drug-resistant tumours
  • Production details: Cells were cultured in vitro in increasing concentrations of either cisplatin or carboplatin until an increase in IC50 between two and ten-fold was achieved to reflect the resistance observed in human patients

Handling

  • Growth medium: RPMI containing 10% FBS and 1% penicillin/streptomycin
  • Temperature: 37° C
  • Cultured in antibiotics: Streptomycin and Penicillin
  • Mycoplasma free: Yes
  • Str profiling: 16 locus STR verification (DNA Diagnostics Centre, London, UK: June 2015?February 2016 and European Collection of Authenticated Cell Lines August 2019)

References

  • J I Hoare et al. 2022. Gynecol Oncol. 167(1):96-106. PMID: 35918200