NUOC-1 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: NUOC-1 Cell Line
• Alternate name: HGSC, high grade mixed ovarian cancer, epithelial ovarian cancer
• Cancer: Gynaecologic cancer
• Cancers detailed: High Grade Ovarian
• Research fields: Cancer;Drug development
• Tool sub type: Continuous
• Parental cell: Ascites of chemotherapy naive patient
• Organism: Human
• Tissue: Ovary
• Disease: Cancer
• Growth properties: Doubling time approximately 58 hours
• Model: Cancer Model
• Conditional: No
• Description: Epithelial ovarian cancer is diagnosed at late disease stage in more than 70% of patients. Ovarian cancer is the leading cause of gynaecological cancer mortality worldwide with a 5-year overall survival of 3039%. It has long been recognised by clinicians that ovarian cancer is a set of heterogeneous diseases. NUOC-1 cell line represents a novel ovarian cancer cell line derived from the ascites of a chemotherapy nave patient with a primary mixed endometrioid/ clear cell/ high grade serous ovarian cancer. NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype
• Production details: Ascites were collected from patients undergoing surgery for ovarian cancer. 20 ml of ascites was added to 20 ml of warmed culture medium in T75 flask, and incubated at 37Â?‚°C, 5% CO2, 95% humidified air. The medium was aspirated and 13 ml of warmed fresh medium was replaced on day 3 to 5. The medium was replaced every 4 to 5 days until the cells approached confluence
• Biosafety level: 1

Target Details

• Target: Ovarian cancer

Applications

• Application notes: NUOC-1 cells grow as an adherent monolayer. The cells are TP53 wildtype, positive for PTEN, HER2 and HER3 expression but negative for oestrogen, progesterone and androgen receptor expression. NUOC-1 cells are competent in homologous recombination and non-homologous end joining, but base excision repair defective. Karyotype analysis demonstrated a complex tetraploid karyotype

Handling

• Format: Frozen
• Growth medium: RPMI 1640 medium supplemented with 20% FCS, 20 mM L-glutamine and 1% penicillin and streptomycin
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

References

• McCormick et al. 2017. Oncotarget. 8(16):26832-26844. PMID: 28460465.
• Functional characterisation of a novel ovarian cancer cell line, NUOC-1.