Cat. #156413
NHE-2 expressing PS120 cell line
Cat. #: 156413
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Disease: Na+ absorption and diarrhea
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Mark Donowitz
Institute: Johns Hopkins University
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: NHE-2 expressing PS120 cell line
- Research fields: Cell signaling and signal transduction;Drug development
- Parental cell: CCL39
- Disease: Na+ absorption and diarrhea
- Description: An isoform of the Na+/H+ exchanger, NHE-2, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:ÄË?Â? NHE-2 can be used to study Na+ absorption and function for treatment of diarrhea when NHE-3 is inhibited by cAMP/cholera toxin
Target Details
- Target: Na+/H+ exchanger
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
