Cat. #156413

NHE-2 expressing PS120 cell line

Name: NHE-2 expressing PS120 cell line
SKU: 156413
Availability: InStock

Cat. #: 156413

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Disease: Na+ absorption and diarrhea

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

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Contributor

Inventor: Mark Donowitz

Institute: Johns Hopkins University

Tool Details

Target Details

Handling

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

Name: NHE-2 expressing PS120 cell line
Cancer type: Lung cancer
Research fields: Cell signaling and signal transduction;Drug development
Parental cell: CCL39
Disease: Na+ absorption and diarrhea
Description: An isoform of the Na+/H+ exchanger, NHE-2, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:ÄË?Â?Â NHE-2 can be used to study Na+ absorption and function for treatment of diarrhea when NHE-3 is inhibited by cAMP/cholera toxin

Target Details

Target: Na+/H+ exchanger

Handling

Format: Frozen
Unit size: 1x10^6 cells / vial
Shipping conditions: Dry ice