Cat. #162230
NBF0LN5-712AL Cell Line
Cat. #: 162230
Availability: 8-10 weeks
Organism: Mouse
Tissue: Skin (lymph node metastasis)
Disease: Melanoma
Model: Tumorigenic cell line; syngeneic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Nathan E. Reticker-Flynn
Institute: Stanford University
Primary Citation: Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: NBF0LN5-712AL Cell Line
- Cancer: Melanoma
- Cancers detailed: Melanoma
- Parental cell: B16-F0
- Organism: Mouse
- Gender: Female
- Tissue: Skin (lymph node metastasis)
- Disease: Melanoma
- Morphology: Mixed morphology that is predominantly spindle-shaped
- Model: Tumorigenic cell line; syngeneic
- Model description: A luciferase transgene was integrated into the B16-F0 parental line, but is no loger expressed in LN metastatic progeny. WES suggests the presence of Braf and Pten mutations.
- Crispr: No
- Description: Fifth generation cell line derived from an ipsilateral axillary lymph node metastasis of a fourth-generation line
- Application: Syngeneic transplantable models of melanoma metastasis
- Production details: Line was isolated from an axillary lymph node metastasis after roughly one month of growth of a LN4 tumor
- Biosafety level: 1
Target Details
- Target background: B16-F0 parental cells
Applications
- Application: Syngeneic transplantable models of melanoma metastasis
Handling
- Growth medium: DMEM (high glucose) + 10% FBS + 4mM L-glutamine (or GlutaMAX) + 1% Pen/Strep (optional)
- Temperature: 37C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Storage medium: DMEM, 50% FBS, 10% DMSO
- Storage conditions: Liquid Nitrogen
- Subculture routine: See product datasheet
- Cultured in antibiotics: Penicillin, Streptomycin
- Mycoplasma free: Yes
- Str profiling: STR profiling has not been performed on the lines, but that of the parental is available at: https://www.cellosaurus.org/CVCL_0604
Related Tools
- Related tools: NBF0LN1-18IL Cell Line, NBF0LN2-262IL Cell Line, NBF0LN3-384IR Cell Line, NBF0LN4-521BL Cell Line, NBF0LN6-987AL Cell Line, NBF0LN7-1120BL Cell Line, NBF0LN8-1205BL Cell Line, NBF0LN9-1315BL Cell Line
References
- Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247
