NBF0LN6-987AL Cell Line

Sixth generation cell line derived from an ipsilateral axillary lymph node metastasis of a fifth-generation line

Contributors

Inventor Institute
Nathan E. Reticker-Flynn Stanford University
Cat. #: 162231
Cancer types: Melanoma
Organism: Mouse
Tissue: Skin (lymph node metastasis)
Gender: Female
Model: Tumorigenic cell line; syngeneic
Morphology: Mixed morphology that is predominantly spindle-shaped
Cancer Types In Detail: Melanoma
Primary citation: Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247
Product description: Sixth generation cell line derived from an ipsilateral axillary lymph node metastasis of a fifth-generation line
Gender: Female
CRISPR: No
Production details: Line was isolated from an axillary lymph node metastasis after roughly one month of growth of a LN5 tumor
Parental cell line: B16-F0
Model description: A luciferase transgene was integrated into the B16-F0 parental line, but is no loger expressed in LN metastatic progeny. WES suggests the presence of Braf and Pten mutations.
disease: Melanoma
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM (high glucose) + 10% FBS + 4mM L-glutamine (or GlutaMAX) + 1% Pen/Strep (optional)
Subculture routine: See product datasheet
Temperature: 37C
Atmosphere: 5% CO2
Storage medium: DMEM, 50% FBS, 10% DMSO
Mycoplasma free: Yes
Biosafety level: 1
References:

Reticker-Flynn, N.E., et al. (2022). 185(11):1924-1942.e23. PMID: 35525247

Related Tools for Cell lines

Cat. # Tool Name
151463 Omega-E Packaging Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151558 MEF Trex1 KO Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool
151629 MEF Raf1 FF/FF KI Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.