Melan-Gnas1 cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Melan-Gnas1 cell line
• Cancer: N/A
• Research fields: Tissue-specific biology
• Organism: Mouse
• Gender: Unspecified
• Tissue: Skin
• Disease: Non-cancer
• Morphology: Small, oval-fusiform
• Growth properties: Adherent
• Model: Immortalised non-cancerous cell lines
• Crispr: No
• Conditional: No
• Description: The melan-Gnas1, -Gnas2 and Gnas-3 cell lines are immortal melanocytes derived from Gnas-mutant Ink4aArf -/- C3H/C57BL/6J OlaHsd mice. The Gnas gene encodes the Gαs protein, a subunit of the heterotrimeric G-protein complex that activates adenylate cyclase and increases cAMP production. Mutations in the human GNAS gene are found in pituitary tumours and result in constitutively activated Gαs. Somatic gain-of-function mutations in GNAS are associated with McCune-Albright Syndrome, a rare disorder characterised by fibrous dysplasia of the bone, various endocrinopathies and hyperpigmented patches of skin. These murine melanocytes are hyperpigmented compared to littermate control lines. These melanocytes are a useful tool for studies of pigmentation genetics and for the effects of constitutively active cAMP signalling. Littermate control lines (melan-a9, -a10 and -a11) are also available from CancerTools.org.
• Application: Melanocyte cell biology
• Biosafety level: BSL1

Applications

• Application: Melanocyte cell biology

Handling

• Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
• Storage conditions: liquid N2

References

• Unpublished