MDA-MB-231/EGFP_Cytochrome c

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MDA-MB-231/EGFP_Cytochrome c
• Cancer: Breast cancer
• Cancers detailed: Breast cancer
• Research fields: Cancer;Disease (not cancer);Metabolism
• Parental cell: MDA-MB-231
• Organism: Human
• Gender: Female
• Tissue: Breast
• Disease: Cancer
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Transgenic
• Crispr: No
• Conditional: No
• Description: The product is a human cervical carcinoma cell line MDA-MB-231, transfected to stably express the protein cytochrome c (cyt-c) tagged with EGFP. The EGFP-tag enables easy identification of the cyt-c release in live cells upon apoptotic induction. The cell line can be utilized for studying the apoptosis process and to screen of potential candidate drugs that induce apoptosis.

Handling

• Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or Leibovitz's L15 with 2mM L-Glutamine and 10% Fetal Bovine serum.
• Temperature: 37° C
• Atmosphere: 5% CO2
• Shipping conditions: Dry Ice
• Storage medium: Growth medium + 10% DMSO
• Storage conditions: Liquid Nitrogen
• Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
• Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .
• Cultured in antibiotics: No

References

• Srinivas KP, et.al. PMID:26992219