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#162292

mCherry HSF-1 + EmGFP-Trim28 expressing U-2 OS cell line

Cat. #162292

mCherry HSF-1 + EmGFP-Trim28 expressing U-2 OS cell line

Cat. #: 162292

Organism: Human

Tissue: Bone

Disease: Cancer

Model: Transgenic

£575.00

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Contributor

Inventor: Petr Muller

Institute: Masaryk Memorial Cancer Institute

Tool Details
Target Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: mCherry HSF-1 + EmGFP-Trim28 expressing U-2 OS cell line
  • Parental cell: U-2 OS
  • Clone: U2OS 1331 + 1401-G8
  • Organism: Human
  • Gender: Female
  • Tissue: Bone
  • Donor: 15 years old, white
  • Disease: Cancer
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Transgenic
  • Model description: Expression of fluorescently mCherry tagged Heat Shock Transcription Factor 1 (HSF1) and the Tripartite motif-containing 28 (TRIM28) with an Emerald Green Fluorescent Protein (EmGFP) tag
  • Crispr: No
  • Conditional: No
  • Description: U-2 OS cell line expressing fluorescently mCherry tagged Heat Shock Transcription Factor 1 (HSF1) used to study the role of HSF1 and assess proteotoxic stress by live cell imaging and the Tripartite motif-containing 28 (TRIM28) with an Emerald Green Fluorescent Protein (EmGFP) tag, used to study the proteotoxic stress by live cell imaging
  • Application: Cell culture, live cell imaging
  • Biosafety level: BSL-2

Target Details

  • Target: HSF-1, TRIM28

Applications

  • Application: Cell culture, live cell imaging

Handling

  • Format: Frozen
  • Atmosphere: humidified CO2 atmosphere
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: Yes, penicillin/streptomycin

References

  • Unpublished

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