MCF7/TAMR-1 Cell Line

Tamoxifen resistant cell line which can be used to discover alternative treatments for breast cancer and understand the signalling pathways involved in tamoxifen resistance.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152089
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer;Breast cancer
Organism: Human
Tissue: Breast
Gender: Female
Model: Tumour line
Growth properties: Adherent
Primary citation: Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513
Alternate name: MCF-7/TAMR-1; MCF7/TAMR-1; MCF7 TAMR-1; MCF-7/TAM(R)-1; MCF7-TAMR; TAMR-1; TamR-1; TamR1; AL-1
Product description: MCF7/TAMR-1 cell line is a stable tamoxifen-resistant subline. This cell line has been established in tissue culture after long term treatment with 1 uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat. This cell line was produced from the parental cell line MCF7/S0.5, as a model cell system to study the effects of tamoxifen resistant cancer growth. MCF7/TAMR-1 cells are oestrogen receptor positive and progesterone receptor negative. They are growth inhibited by the pure antioestrogen fulvestrant.Previous applications of this cell line include treatment with steroidal antiestrogens. MCF7/TAMR-1 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance. Additionally MCF/TAMR-1 can aid in identifying new predictive markers for response to hormonal therapy.
Gender: Female
CRISPR: No
Conditional: Yes
Production details: The parental cell line for the MCF7/TAMR-1 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-1 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. The establishment of the MCF7/TAMR-1 cell line, originally named AL-1, was first described in Lykkesfeldt et al (1986). Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is le…
Additional notes: During the establishment process the treatment of MCF7/S0.5 cells with tamoxifen was started in passage 351. Few colonies of cells survived the treatment and after 28 days of tamoxifen treatment, tamoxifen was omitted from the medium for 22 days. After 19 passages without tamoxifen (passage 372) the cells underwent a second treatment with tamoxifen which initially reduced cell growth rate, but around 390-400 the growth rate of the tamoxifen resistant cell lines was close to the growth rate of…
Cellosaurus ID: CVCL_M436
Receptors of note: Oestrogen receptor positive and progesterone receptor negative
Parental cell line: MCF7/S0.5 Cell Line
disease: Cancer;Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol red-free DMEM/F-12 containing 1% Fetal bovine serum, 2 mM Glutamax and 6 ng/ml Insulin. To maintain high-level resistance, the medium should be supplemented with 1 uM Tamoxifen
Temperature: 37° C
Atmosphere: 5% CO2
Recommended controls: MCF7-S0.5 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Lee et al. 2018. Autophagy. 14(5):812-824. PMID: 29130361

Martin et al. 2017. Nat Commun. 8(1):1865. PMID: 29192207

Guney Eskiler et al. 2016. Cell Biol Toxicol. :. PMID: 27585693

Joshi et al. 2016. Oncotarget. :. PMID: 27528030

Leclercq et al. 2016. J Recept Signal Transduct Res. :1-18. PMID: 27400858

Guest et al. 2016. PLoS One. 11(6):e0157397. PMID: 27308830

Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755

Elias et al. 2015. Oncogene. 34(15):1919-27. PMID: 24882577

Thrane et al. 2015. Oncogene. 34:4199–4210. PMID: 25362855

Nass et al. 2014. PLoS One. 9(7):e101473. PMID: 24983248

Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470

Cutrupi et al. 2012. Oncogene. 31(40):4353-61. PMID: 22249258

Plaza-Menacho et al. 2010. Oncogene. 29(33):4648-57. PMID: 20531297

Lykkesfeldt et al. 1994. Cancer Res. 54(6):1587-95. PMID: 8137264

Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513

Citations And Reviews

Related Tools for Cell lines

Cat. # Tool Name
151463 Omega-E Packaging Cell Line Key Info View Tool
151480 HEK293A EGFP-LC3 Cell Line Key Info View Tool
151484 2fTGH Cell Line Key Info View Tool
151488 B6.1.1 Mouse ES Cell Line Key Info View Tool
151559 MEF UNG KO Cell Line Key Info View Tool

Related News

UM-UC-10 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
Cell Lines

Popular cell lines now available only from CancerTools.org

Read More
Cell Lines

New cell lines: murine melanocyte and melanoblast cell lines from Dr. Elena Sviderskaya

Read More
Image Reference: 2x PTEN -/- mouse prostate cancer cell lines (MuCap system). Histology staining of syngraft tumours (Van Duijn et al., 2018).
Cell Lines

38 new prostate cancer cell lines

Read More

Tool enquiry

Please ensure you use your organisation email address rather than personal where possible, as this helps us locate your organisation in our system faster.

Please note we may take up to three days to respond to your enquiry.

CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.