MCF7/S9 Cell Line

The MCF7/S9 cell line was established to grow in serum free medium to enable studies of the effect of estrogens and antiestrogens under conditions without influence of estrogenic compounds supplied via the serum content in the growth medium.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society, Denmark
Cat. #: 152602
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Growth properties: Adherent: Grows in serum free medium. Estrogen and progesterone receptor positive. Estrogen and antiestrogen responsive.
Cancer Types In Detail: Breast cancer
Primary citation: Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-478. PMID: 12732292.
Alternate name: MCF-7/S9; S9
Product description: MCF-7/S9 cell line is an oestrogen-responsive (not oestrogen-dependent) breast cancel model. This model is derived from MCF-7, a human breast carcinoma line with epithelial like morphology. The MCF-7/S9 cell line was established to grow in serum free medium. Previous findings of the MCF-7/S9 cell line include antioestrogens, specifically tamoxifen, fulvestrant and acolbifene exert a significant direct growth-inhibitory effect. This model enables investigation of the effect of oestrogens and antioestrogens on tumour cell growth under conditions without influence of oestrogenic compounds supplied via the serum content in the growth medium.
Gender: Female
Conditional: No
Production details: MCF7/S9 has been established by gradually adapting MCF7 cells to grow at a low serum concentration before transfer to serum-free medium. Since passage no. 387, the cell line has been propagated in phenol red-free DMEM/F12 (1:1) without addition of serum or any growth factor (standard medium for MCF7/S9), in T-flasks coated with collagen. The MCF7/S9 was subcultured once a week with a split ratio of approximately 1:10 and growth medium was changed every second or third day.
Cellosaurus ID: CVCL_AS09
Parental cell line: MCF7
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 2.0 mM Glutamax, 10 ug/ml Transferrin, 0.1 mg/ml bovine serum albumin, culture flasks were coated with collagen IV.
Recommended controls: MCF7 parental line
References:

Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-478. PMID: 12732292.

Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.

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