MCF7/S9 Cell Line

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Cat. #152602

MCF7/S9 Cell Line

Cat. #: 152602

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Breast

Disease: Cancer


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Inventor: Anne Lykkesfeldt

Institute: Danish Cancer Society, Denmark

Primary Citation: Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-478. PMID: 12732292.

Tool Details
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Tool Details


  • Name: MCF7/S9 Cell Line
  • Alternate name: MCF-7/S9; S9
  • Cancer: Breast cancer
  • Cancers detailed: Breast cancer
  • Research fields: Cancer;Drug development
  • Tool sub type: Continuous
  • Parental cell: MCF7
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Disease: Cancer
  • Growth properties: Grow in serum free medium. Estrogen and progesterone receptor positive. Estrogen and antiestrogen responsive.
  • Conditional: No
  • Description: MCF-7/S9 cell line is an oestrogen-responsive (not oestrogen-dependent) breast cancel model. This model is derived from MCF-7, a human breast carcinoma line with epithelial like morphology. The MCF-7/S9 cell line was established to grow in serum free medium. Previous findings of the MCF-7/S9 cell line include antioestrogens, specifically tamoxifen, fulvestrant and acolbifene exert a significant direct growth-inhibitory effect. This model enables investigation of the effect of oestrogens and antioestrogens on tumour cell growth under conditions without influence of oestrogenic compounds supplied via the serum content in the growth medium.
  • Application: Evaluation of antioestrogens inhibitory effects
  • Production details: MCF7/S9 has been established by gradually adapting MCF7 cells to grow at a low serum concentration before transfer to serum-free medium. Since passage no. 387, the cell line has been propagated in phenol red-free DMEM/F12 (1:1) without addition of serum or any growth factor (standard medium for MCF7/S9), in T-flasks coated with collagen. The MCF7/S9 was subcultured once a week with a split ratio of approximately 1:10 and growth medium was changed every second or third day.
  • Recommended controls: MCF7 parental line
  • Cellosaurus id: CVCL_AS09


  • Application: Evaluation of antioestrogens inhibitory effects


  • Format: Frozen
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 2.0 mM Glutamax, 10 ug/ml Transferrin, 0.1 mg/ml bovine serum albumin, culture flasks were coated with collagen IV.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: MCF7/S0.5 Cell Line


  • Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-478. PMID: 12732292.
  • Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.