#152090

MCF7/S0.5 Cell Line

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Cat. #152090

MCF7/S0.5 Cell Line

Cat. #: 152090

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Breast

Disease: Cancer

Model: Tumour line

£800.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Anne Lykkesfeldt

Institute: Danish Cancer Society

Primary Citation: Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: MCF7/S0.5 Cell Line
  • Alternate name: MCF7/S0.5; MCF-7/S0.5; MCF7 S0.5
  • Cancer: Breast cancer
  • Cancers detailed: Breast cancer
  • Research fields: Cancer;Drug development
  • Tool sub type: Continuous
  • Parental cell: MCF7
  • Organism: Human
  • Gender: Female
  • Tissue: Breast
  • Donor: Female, 69 y.o., Caucasian
  • Disease: Cancer
  • Morphology: Epithelial; Adherent polygonal epithelia
  • Growth properties: Adherent
  • Model: Tumour line
  • Crispr: No
  • Conditional: Yes
  • Receptors of note: Oestrogen receptor and progesterone receptors
  • Description: The MCF7/S0.5 human breast cancer cell line has been established to grow at low serum. The presence of hormones in the serum required in tissue culture media complicates the demonstration of specific hormone and antihormone effects. Methods to overcome this have included charcoal-treated serum and serum-free medium. However, both methods have serious drawbacks. Therefore, the MCF-7/S0.5 cell line has been adapted to long-term growth at 0.5 % fetal bovine serum in order to reduce the oestroge...
  • Application: Hormone and anti-hormone effects studies; Studies of the effect of oestrogens; Estradiol and tamoxifen effects studies
  • Production details: This MCF7 subline was established buy stepwise reduction in the serum concentration from 5% fetal calf serum to 0.5 % in growth medium consisting of DMEM: Ham's F-12 medium (1:1) supplemented with 2mM glutamine and insulin (6ng/ml)
  • Biosafety level: 1
  • Cellosaurus id: CVCL_1D47

Target Details

  • Target: Oestrogen receptor

Applications

  • Application: Hormone and anti-hormone effects studies; Studies of the effect of oestrogens; Estradiol and tamoxifen effects studies

Handling

  • Format: Frozen
  • Passage number: Passage 408 (AL2542, AL2543)
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 1% Fetal calf serum 2.5 mM Glutamax and 6 ng/ ml Insulin
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Subculture routine: Split at 70-80% confluence: 1:3 to 1:6 (seeding at 2-4x104 cells/cm2) using 0.05% trypsin or trypsin or 0.05% trypsin/EDTA solution. Change the media every 2-3 days
  • Mycoplasma free: Yes

Related Tools

  • Related tools: MCF7/TAMR-1 Human Breast Cancer Cell Line ; MCF7/TAMR-4 Cell Line ; MCF7/TAMR-7 Cell line ; MCF7/TAMR-8 Cell Line ; MCF7/S9 Cell Line

References

  • Joshi et al. 2016. Oncotarget. :. PMID: 27528030
  • Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418
  • Thomsen et al. 2015. Breast Cancer Res Treat. :. PMID: 26585578
  • Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755
  • Sonne-Hansen et al. 2005. J Steroid Biochem Mol Biol. 93(1):25-34. PMID: 15748829
  • Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159
  • Madsen et. al. 1995. Mol Cell Endocrinol 109(2):197-207. PMID: 7664983
  • Lykkesfeldt et al. 1994. Cancer Res. 54(6)...