MCF7/S0.5 Cell Line
The anti-oestrogen sensitive MCF-7/S0.5 line is a low serum adapted variant of the MCF-7 human breast cancer cell line with enhanced growth capabilities.
Contributors
| Inventor | Institute |
|---|---|
| Anne Lykkesfeldt | Danish Cancer Society |
| Cat. #: | 152090 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Tumour line |
| Morphology: | Epithelial; Adherent polygonal epithelia |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Breast cancer |
| Primary citation: | Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856 |
| Alternate name: | MCF7/S0.5; MCF-7/S0.5; MCF7 S0.5 |
|---|---|
| Product description: | The MCF7/S0.5 cell line has been established to grow at low serum. the MCF-7/S0.5 cell line has been adapted to long-term growth at 0.5 % fetal bovine serum in order to reduce the oestrogen supply via serum and to enable oestrogen receptor-mediated growth inhibition with the antiestrogen tamoxifen._x000D_ _x000D_ The MCF7/S0.5 cells are also useful for studies of effect of oestrogens, if they, instead of 1 % fetal calf serum, are cultivated with 10% newborn calf serum which contains very low oestrogenic activity. |
| Gender: | Female |
| CRISPR: | No |
| Conditional: | Yes |
| Production details: | This MCF7 subline was established buy stepwise reduction in the serum concentration from 5% fetal calf serum to 0.5 % in growth medium consisting of DMEM: Ham's F-12 medium (1:1) supplemented with 2mM glutamine and insulin (6ng/ml) |
| Cellosaurus ID: | CVCL_1D47 |
| Receptors of note: | Oestrogen receptor and progesterone receptors |
| Parental cell line: | MCF7 |
| Disease: | Cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Phenol-red-free DMEM/F12 medium supplemented with 1% Fetal calf serum 2.5 mM Glutamax and 6 ng/ ml Insulin |
| Subculture routine: | Split at 70-80% confluence: 1:3 to 1:6 (seeding at 2-4×104 cells/cm2) using 0.05% trypsin or trypsin or 0.05% trypsin/EDTA solution. Change the media every 2-3 days |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Joshi et al. 2016. Oncotarget. :. PMID: 27528030 Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418 Thomsen et al. 2015. Breast Cancer Res Treat. :. PMID: 26585578 Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755 Sonne-Hansen et al. 2005. J Steroid Biochem Mol Biol. 93(1):25-34. PMID: 15748829 Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159 Madsen et. al. 1995. Mol Cell Endocrinol 109(2):197-207. PMID: 7664983 Lykkesfeldt et al. 1994. Cancer Res. 54(6)… |
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Product Images
MCF-7/S0.5 cells
MCF-7/S0.5 cells stained for oestrogen receptor expression
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