MCF7/S0.5 Cell Line

The MCF7/S0.5 cell line has been established to grow at low serum. the MCF-7/S0.5 cell line has been adapted to long-term growth at 0.5 % fetal bovine serum in order to reduce the oestrogen supply via serum and to enable oestrogen receptor-mediated growth inhibition with the antiestrogen tamoxifen._x000D_ _x000D_ The MCF7/S0.5 cells are also useful for studies of effect of oestrogens, if they, instead of 1 % fetal calf serum, are cultivated with 10% newborn calf serum which contains very low oestrogenic activity.

Contributors

Inventor Institute
Anne Lykkesfeldt Danish Cancer Society
Cat. #: 152090
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Gender: Female
Model: Tumour line
Morphology: Epithelial; Adherent polygonal epithelia
Growth properties: Adherent
Cancer Types In Detail: Breast cancer
Primary citation: Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856
Alternate name: MCF7/S0.5; MCF-7/S0.5; MCF7 S0.5
Product description: The MCF7/S0.5 human breast cancer cell line has been established to grow at low serum. The presence of hormones in the serum required in tissue culture media complicates the demonstration of specific hormone and antihormone effects. Methods to overcome this have included charcoal-treated serum and serum-free medium. However, both methods have serious drawbacks. Therefore, the MCF-7/S0.5 cell line has been adapted to long-term growth at 0.5 % fetal bovine serum in order to reduce the oestrogen supply via serum and to enable oestrogen receptor-mediated growth inhibition with the anti-oestrogens like tamoxifen. The MCF7/S0.5 cells are also useful for studies investigating the effects of oestrogens, if they, instead of 1% fetal calf serum, are cultivated with 10% newborn calf serum which contains very low oestrogenic activity. MCF7/S0.5 cells express both oestrogen and progesterone receptors. Many tamoxifen, fulvestrant, letrozole, anastrozole, and exemestane resistant cell lines have been derived from MCF7/S0.5 and so this line can be utilized as a parental sensitive control to its derivative resistant cell lines in parallel experiments.
Gender: Female
CRISPR: No
Conditional: Yes
Production details: This MCF7 subline was established buy stepwise reduction in the serum concentration from 5% fetal calf serum to 0.5 % in growth medium consisting of DMEM: Ham's F-12 medium (1:1) supplemented with 2mM glutamine and insulin (6ng/ml)
Cellosaurus ID: CVCL_1D47
Receptors of note: Oestrogen receptor and progesterone receptors
Parental cell line: MCF7
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 1% Fetal calf serum 2.5 mM Glutamax and 6 ng/ ml Insulin
Subculture routine: Split at 70-80% confluence: 1:3 to 1:6 (seeding at 2-4×104 cells/cm2) using 0.05% trypsin or trypsin or 0.05% trypsin/EDTA solution. Change the media every 2-3 days
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Joshi et al. 2016. Oncotarget. :. PMID: 27528030

Alves et al. 2016. Clin Cancer Res. :. PMID: 27252418

Elias et al. 2015. Oncogene. 34:1919–1927. PMID: 24882577

Thomsen et al. 2015. Breast Cancer Res Treat. :. PMID: 26585578

Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755

Sonne-Hansen et al. 2005. J Steroid Biochem Mol Biol. 93(1):25-34. PMID: 15748829

Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159

Madsen et. al. 1995. Mol Cell Endocrinol 109(2):197-207. PMID: 7664983

Lykkesfeldt et al. 1994. Cancer Res. 54(6):1587-95. PMID: 8137264

Lykkesfeldt et al. 1989. Mol Cell Endocrinol. 62(2):287-96. PMID: 2744230

Lykkesfeldt et al. 1986. Br J Cancer. 53(1):29-35. PMID: 3947513

Briand et al. 1984. Cancer Res. 44(3):1114-9. PMID: 6362856.

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