MCF7aro Let-R Cell Line

MCF7aro Let-R Cell Line is a human cell line model of aromatase inhibitor resistant breast cancer.

Contributors

Inventor Institute
Marie McIlroy Royal College of Surgeons Ireland (RCSI)
Cat. #: 151781
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer;Breast cancer
Organism: Human
Tissue: Breast
Gender: Male
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Breast cancer;Breast cancer
Product description: MCF7aro Let-R Cell Line is a human cell line model of aromatase inhibitor resistant breast cancer.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: Letrozole (aromatase inhibitor) sensitive cells (MCF-7aro) were developed by stable transfection of the aromatase gene (CYP19); Letrozole-resistant cells (MCF-7aro Let-R) were created by long-term treatment of aromatase-expressing MCF7 cells (MCF7aro) with letrozole.
Cellosaurus ID: CVCL_W348
Parental cell line: MCF7
disease: Cancer;Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: They are cultured in phenol red free Eagle's Minimum Essential Medium (Phenol-red free MEM) (Sigma Aldrich), 10% charcoal dextran stripped FCS (CDS-FCS), 1% Pen-Strep (Sigma Aldrich), 2 mM L-Glutamine (Sigma Aldrich) and 200 ?„?žg/ml G418 disulfate salt (Geneticin) (Sigma Aldrich), 2.5-8 M androstenedione and 10-6 M letrozole.
Mycoplasma free: Yes
Biosafety level: 1
References:

Cellosaurus MCF-7aro Let-R (CVCL_W348)

O'Hara et al. 2012. Clin Cancer Res. 18(12):3305-15. PMID: 22550166.

AIB1:ERa transcriptional activity is selectively enhanced in aromatase inhibitor-resistant breast cancer cells.

McIlroy et al. 2010. Cancer Res. 70(4):1585-94. PMID: 20145129.

Interaction of developmental transcription factor HOXC11 with steroid receptor coactivator SRC-1 mediates resistance to endocrine therapy in breast cancer [corrected].

Citations And Reviews

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