MB49-luc Cell Line

MB49-luc is an aggressive, bioluminescent orthotopic bladder cancer model stably expressing luciferase, derived from the well-established MB49 cell line. The bioluminescence can be detected by in vivo imaging and offers…

Contributors

Inventor Institute
Jeffrey Schlom, PhD National Cancer Institute
Cat. #: 161579
Tool sub type: 10XUAS IVS p10
Organism: Mouse
Tissue: Bladder
Gender: Male
Model: Tumourigenic line
Morphology: Epithelial cells
Growth properties: Adherent
Primary citation: Vandeveer et al. Cancer Immunol Res. 2016 May, 4(5):452-62. PMID: 26921031
Alternate name: MB49-luciferase, MB49-luc, MB-49-Luc, MB49luc, MB-49-luc, MB49-LUC, MB49 Luciferase (firefly) Cells
Product description: MB49-luc is an aggressive, bioluminescent orthotopic bladder cancer model stably expressing luciferase, derived from the well-established MB49 cell line. The bioluminescence can be detected by in vivo imaging and offers a readout for tumour take, growth and reduction. Similarly to its parental cell line MB49 (Cat. #: 153368), it forms tumours when injected subcutaneously or orthotopically into mouse bladders. Specifically, the orthotopic intravesical bladder tumour model based on MB49-luc offers a system to study immune-related factors involved in non-muscle invasive, non-metastatic bladder tumour growth, including anti-tumour effects of treatments such as immune checkpoint inhibitors. It also provides a bladder cancer model to study mechanisms of immunotherapy non-responders, to help identifying effective immune-based combination therapies and PD-L1 function within a tumour microenvironment devoid of T cells.
Gender: Male
CRISPR: No
Conditional: No
Production details: Parental MB49 cells transfected with a pSELECT-zeo-LucSh plasmid using Lipofectamine (InvivoGen) for luciferase expression detected by in vivo imaging
Parental cell line: MB49 Cell Line
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: DMEM with 1 mM sodium pyruvate, 2 mM glutamine, 10% FBS and 1x Penicillin/Streptomycin (optional). Zeocin (200 µg/ml) may be added to confirm stable expression and retention of the transfected plasmid.
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Domingos-Pereira et al. Int J Mol Sci. 2022. 24(1):123. PMID: 36613562.

Vandeveer et al. Cancer Immunol Res. 2016. 4(5):452-462. PMID: 26921031.

Images

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MB49 Luc, figure adapted from Vandeveer et al 2015, Figure 2A-B, (A) Bioluminescence images and total flux radiance measurements of MB49luc bladder tumors on day 8 post tumor instillation. Mice received 400 µg of either the isotype control Ig or avelumab on days 9, 12, and 15. (B) Day 21 bioluminescence images and radiance measurements of those mice.(6)

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