MB49-luc Cell Line

Luciferase derivative of gold standard murine urothelial carcinoma model MB49. Used for real-time tracking of tumour burden.

Contributors

Inventor Institute
Jeffrey Schlom, PhD National Cancer Institute
Cat. #: 161579
Cancer types: Bladder cancer
Research Fields: Cancer
Organism: Mouse
Tissue: Bladder
Gender: Male
Model: Tumourigenic line
Morphology: Epithelial cells
Growth properties: Adherent
Cancer Types In Detail: Bladder
Primary citation: Vandeveer et al. Cancer Immunol Res. 2016 May, 4(5):452-62. PMID: 26921031
Alternate name: MB49-luciferase, MB49-luc, MB-49-Luc, MB49luc, MB-49-luc, MB49-LUC, MB49 Luciferase (firefly) Cells
Product description: <span class="excerpt_part">MB49-luc is an aggressive, bioluminescent orthotopic bladder cancer model stably expressing luciferase, derived from the well-established MB49 cell line. The bioluminescence can be detected by in vivo imaging and offers…</span>
Gender: Male
CRISPR: No
Conditional: No
Production details: Parental MB49 cells transfected with a pSELECT-zeo-LucSh plasmid using Lipofectamine (InvivoGen) for luciferase expression detected by in vivo imaging
Parental cell line: MB49 Cell Line
Disease: Cancer
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Growth medium: Dulbecco's Modified Eagle Medium (DMEM) with 10% heat-inactivated foetal bovine serum supplemented with 1 mM nonessential amino acids, 1 mM sodium pyruvate, 2 mM glutamine, and penicillin/streptomycin (100 U/mL).
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Domingos-Pereira et al. Int J Mol Sci. 2022. 24(1):123. PMID: 36613562.

Vandeveer et al. Cancer Immunol Res. 2016. 4(5):452-462. PMID: 26921031.

Images

View Gallery
MB49 Luc, figure adapted from Vandeveer et al 2015, Figure 2A-B, (A) Bioluminescence images and total flux radiance measurements of MB49luc bladder tumors on day 8 post tumor instillation. Mice received 400 µg of either the isotype control Ig or avelumab on days 9, 12, and 15. (B) Day 21 bioluminescence images and radiance measurements of those mice.(6)

Citations And Reviews

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