J558L Cell Line secreting OX40L

Cat. #153661

J558L Cell Line secreting OX40L

Cat. #: 153661

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Mouse


This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.


Inventor: Peter Lane

Institute: University of Birmingham

Primary Citation: Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042

Tool Details
Target Details

Tool Details


  • Name: J558L Cell Line secreting OX40L
  • Alternate name: OX4L ligand, CD134, dendritic cells, DCs, Th2, CD525, systemic lupus erythematosus, TXGP1, OX-4L, gp34, TNFSF4, CD4 T cells, cytokine, Tumor necrosis factor receptor superfamily member 4, TNFRSF4
  • Tool sub type: Continuous
  • Parental cell: J558L - mammalian plasmacytoma cell line
  • Organism: Mouse
  • Conditional: No
  • Description: OX40 receptor also known as CD134, a member of the tumor necrosis factor receptor superfamily of receptors, is not constitutively expressed on resting naive T cells. OX40 is activated 24 to 72 hours after binding to its ligand; OX40L, and is a secondary co-stimulatory immune checkpoint molecule. Expression of OX40 is dependent on full activation of the CD28 absent-T cell.
  • Production details: Primers specific to the murine sequence of the OX40L gene were used to generate a cDNA amplicon which was cloned into an expression vector. The vector has resistant markers to ampicillin and histidinol, and also contains promoters and enhancers for murine B-cells and plasma cells. A chimeric IgG1 and OX40 molecule with a 3' splice donor site was generated by subcloning the OX40L amplicon into a human IgG1 expression vector.
  • Biosafety level: 1

Target Details

  • Target: Murine OX40L


  • Format: Frozen
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes


  • Flynn et al. 1998. J Exp Med. 188(2):297-304. PMID: 9670042