#156505

Immortalized Fibroblastic Reticular cell line

Cat. #156505

Immortalized Fibroblastic Reticular cell line

Cat. #: 156505

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Lymph Node

Model: Immortalised Line

£575.00

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Contributor

Inventor: Akira Ono

Institute: University of Michigan

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Immortalized Fibroblastic Reticular cell line
  • Parental cell: Human lymphatic fibroblasts
  • Organism: Human
  • Tissue: Lymph Node
  • Model: Immortalised Line
  • Description: Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection.
  • Production details: Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418.

Handling

  • Format: Frozen
  • Growth medium: The cell line was maintained in fibroblast medium containing 2% FBS, P/S, and fibroblast growth supplement and subsequently selected with 1000â?‚€?‚‰Ă‚?„?žg/ml G418.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Murakami et al. 2018. Nat Commun. 22
  • 9(1):2436. PMID: 2993452