Immortalized Fibroblastic Reticular cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Immortalized Fibroblastic Reticular cell line
• Research fields: Immunology
• Parental cell: Human lymphatic fibroblasts
• Organism: Human
• Tissue: Lymph Node
• Model: Immortalised Line
• Description: Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection.
• Production details: Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418.

Handling

• Format: Frozen
• Growth medium: Fibroblast medium 2 (FM-2), ScienCell Catalog #2331
• Temperature: 37°C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Subculture routine: Seed cells at 80000/ ml or 16000/cm2. Split every 2-3 days, maintain for up to two months post-revival of frozen cells. Please also see detailed protocol within the Product Datasheet in the Documentation section below.

References

• Murakami et al. 2018. Nat Commun. 22:9(1):2436. PMID: 29934525

Documentation

• Product Datasheet