Immortalized Fibroblastic Reticular cell line

Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and […]

Contributors

Inventor Institute
Akira Ono University of Michigan
Cat. #: 156505
Unit size: 1×10^6 cells / vial
Research Fields: Immunology
Organism: Human
Tissue: Lymph Node
Model: Immortalised Line
Product description: Human lymphatic fibroblasts were immortalized by transfecting them with a vector that expresses HPV16 E6 and E7 genes. Primary fibroblastic reticular cells (FRCs) are stromal cells found in secondary lymphoid organs, which are major sites for HIV-1 infection of CD4+ T cells. Using the immortalized FRCs it was shown that FRCs capture HIV-1 particles and facilitate infection of T cells when FRCs contact T cells, increasing HIV-1 spread via trans-infection.
Production details: Immortalized Human lymphatic fibroblasts were generated by transfection of cells with vector pLXSN16E6E7, which encodes HPV E6 and E7, and subsequent selection in fibroblast growth supplement containing G418. A clone was isolated from G418-resistant cells by limiting dilution and cultured in fibroblast growth supplement without G418.
Parental cell line: Human lymphatic fibroblasts
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Fibroblast Medium (FM, 2% FBS) — ScienCell, Cat# 2301 Note: The term "FM-2" used in the original publication refers to Fibroblast medium containing 2% FBS (ScienCell Cat# 2301)
Subculture routine: Seed cells at 80000/ ml or 16000/cm2. Split every 2-3 days, maintain for up to two months post-revival of frozen cells. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37°C
Atmosphere: 5% CO2
References:

Murakami et al. 2018. Nat Commun. 22:9(1):2436. PMID: 29934525

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