Cat. #162301
IDHmut glioma stem-like NCH551b cell line
Cat. #: 162301
Organism: Human
Tissue: Brain
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Christel Herold-Mende
Institute: Heidelberg University
Primary Citation: Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: IDHmut glioma stem-like NCH551b cell line
- Cancer: Brain
- Organism: Human
- Tissue: Brain
- Growth properties: Mixed - adherent and suspension
- Description: Glioma stem-like cell line NCH551b expressing mutant isocitrate dehydrogenase (IDH), used to model low-grade gliomas or secondary glioblastomas.
- Biosafety level: 2
Handling
- Growth medium: DMEM/Ham F-12 supplemented with 1% Penicillin/Streptomcyin, 1% 200mM Glutamine (preferably Glutamax), 10% BIT supplement (PeloBiotech), bFGF and EGF
- Temperature: 37°C
- Atmosphere: 5% CO2
- Storage conditions: FCS-free freezing medium (Synth-a-freeze medium)
References
- Trong et al. 2023. Cells. 12(8).
- Cano-Galiano et al. 2021. Neurooncol Advances. 3(1). PMID: 34250481.
- Ricklefs et al. 2020. Int J Mol Sci. 21(6).
- Trong et al. 2020. Cells. 9(6).
- Biedermann et al. 2019. Cancers. 11(12).
- Trong et al. 2018. Int J Mol Sci. 19(10):2903. PMID: 30257451.
- Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.
- Bunse et al. 2018. Nat Med. 24(8):1192-1203. PMID: 29988124.
- Pusch et al. 2017. Acta Neuropathol. 133(4):629-644. PMID: 28124097.
- Kohanbash et al. 2017. J Clin Invest. 127(4):1425-1437. PMID: 28319047.
