Cat. #151717
HEK293-A GFP-WIPI1 Cell Line
Cat. #: 151717
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Embryo
Disease: Cancer
Model: Transgenic
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sharon Tooze
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HEK293-A GFP-WIPI1 Cell Line
- Research fields: Apoptosis and autophagy;Cancer;Cell biology;Cell signaling and signal transduction;Drug development;Metabolism
- Tool sub type: Continuous
- Parental cell: HEK293-A
- Organism: Human
- Tissue: Embryo
- Disease: Cancer
- Growth properties: Autophagy
- Model: Transgenic
- Description: The HEK293-A GFP-WIPI1 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI1 in background of HEK293-A cell line
- Production details: HEK293-A cells were transfected with GFP-WIPI1 then selcted with G418, and stable, amino acid responsive clones were selected.
- Biosafety level: 1
- Additional notes: HEK293-A cells were obtained under licence from AdVec
- Recommended controls: HEK293-A parental line
- Cellosaurus id: CVCL_5F67
Target Details
- Target: Human WIPI1, GFP
Handling
- Format: Frozen
- Growth medium: DMEM with 10% FCS
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: HEK293-A GFP-WIPI2 Cell Line
References
- Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.
- WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.
