Cat. #140075
GCGR E43 Cell line
Cat. #: 140075
Organism: Human
Tissue: Grade IV Glioblastoma
Model: Primary cell line
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Gillian Morrison, Steven Pollard
Institute: University of Edinburgh
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: GCGR E43 Cell line
- Cancer type: Brain cancer
- Cancers detailed: Glioblastoma
- Organism: Human
- Tissue: Grade IV Glioblastoma
- Morphology: Adherent monolayer with stem-like features (SOX2, NESTIN).
- Growth properties: Adherent
- Model: Primary cell line
- Description: GCGR-E43 is a primary, patient-derived, IDH-wildtype glioblastoma stem-like cell line generated to model Classical subtype GBM in vitro. It exhibits a Classical transcriptional identity, NonMESImm status, and a GBM_RTK_II methylation subclass, growing as an adherent, laminin-dependent monolayer with a doubling time of ~140 hours. GCGR-E43 is tumourigenic in vivo, forming tumours after approximately three months, and is supported by comprehensive molecular profiling, including RNA-seq, WGS, EPIC methylation arrays, STR and CNV data. GCGR-E43 is used to investigate Classical-subtype GBM biology and stem-like cell behaviour, and is suitable for 2D viability assays, 3D spheroid assays, and immunocytochemistry based phenotyping. Its Classical/RTK-II molecular identity makes it a valuable comparator to Proneural and Mesenchymal GCGR lines within the collection
- Application: Used to study glioblastoma stem-like cell biology, Classical subtype signalling, tumourigenicity, and drug response.
- Production details: Derived from surgically resected primary glioblastoma tissue and expanded as an adherent monolayer under laminin-supported condition
- Biosafety level: 2
- Additional notes: Primary cell lines
- Recommended controls: GCGR human neural stem cell lines
Applications
- Application: Used to study glioblastoma stem-like cell biology, Classical subtype signalling, tumourigenicity, and drug response.
Handling
- Format: Frozen
- Growth medium: Serum‑free DMEM/HAMS‑F12 (“Complete + EFL”) supplemented with glucose, NEAA, B27, N2, BSA, beta‑mercaptoethanol, Pen/Strep, and recombinant EGF (10 ng/mL), FGF‑2 (10 ng/mL), plus laminin‑1 (2 ug/mL). Laminin is required for adherent growth.
- Subculture routine: Passage at 80–90% confluence; split 1:4–1:6; detach using Accutase; avoid over‑dilution; replace medium weekly
- Cultured in antibiotics: Pen/Strep
- Characterisation tests: RNA‑seq, methylation array, WGS, copy‑number profiling; STR profile available
