#162350

EYFP CH12F3 cell line

Cat. #162350

EYFP CH12F3 cell line

Cat. #: 162350

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Blood

Disease: Lymphoma

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Bodil Merete Kavli

Institute: Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: EYFP CH12F3 cell line
  • Cancer: Blood cancer
  • Tool sub type: Continuous
  • Organism: Mouse
  • Tissue: Blood
  • Disease: Lymphoma
  • Model: Transgenic
  • Model description: EYFP expression using CRISPR-Cas9
  • Crispr: Yes
  • Description: Mouse B lymphocyte cell line expressing enhanced yellow fluorescent protein
  • Biosafety level: 2

Target Details

  • Target: AID

Handling

  • Format: Frozen
  • Passage number: Mouse B lymphocyte cell line expressing EYFP
  • Growth medium: RPMI culture medium supplemented with 10% heat-inactivated foetal calf serum, 0.03% l-glutamine, 50 M -mercaptoethanol, 1 mM sodium pyruvate, 0.1 mg/ml penicillin/streptomycin, 2.3 g/ml fungizone, and 1.0 mg/ml G419
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Cultured in antibiotics: Yes, penicillin/streptomycin

References

  • Pettersen et al., 2015. DNA Repair (Amst). 25:60-71, PMID: 25486549