#152597

E3 Cell Line

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Cat. #152597

E3 Cell Line

Cat. #: 152597

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Organism: Mouse

Tissue: Breast

Disease: Cancer

Model: Immortalised Line

£575.00

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Contributor

Inventor: Joyce Taylor-Papadimitriou ; Joy Burchell

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: E3 Cell Line
  • Cancer: Gynaecologic cancer
  • Cancers detailed: Mammary
  • Research fields: Cancer;Drug development;Immunology
  • Tool sub type: Continuous
  • Parental cell: 410.4
  • Organism: Mouse
  • Tissue: Breast
  • Disease: Cancer
  • Model: Immortalised Line
  • Description: The E3 cell line was developed to examine the possibility of using the MUC1 gene and its products in active immunization against breast and other carcinomas. The human MUC1 gene codes for a type I membrane glycoprotein that is normally expressed on the apical surface of most glandular epithelial cells, but which is upregulated and under- or differently glycosylated in carcinomas; these differences in glycosylation lead to the exposure of novel epitopes which are not found on the normally processed mucin and can therefore be used as targets.
  • Production details: The E3 cell line was developed from the 410.4 murine mammary cell line by transfection of the hygromycin resistance gene with the MUC1 gene.
  • Cellosaurus id: CVCL_W345

Target Details

  • Target: hMUC1, hygromycin resistance gene

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10S , 400?„?žg/ml hygromycin
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
  • Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.
  • Smith et al. 1999. Immunology. 97(4):648-55. PMID: 10457219.
  • Expression of B7.1 in a MUC1-expressing mouse mammary epithelial tumour cell line inhibits tumorigenicity but does not induce autoimmunity in MUC1 transgenic mice.
  • Lalani et al. 1991. J Biol Chem. 266(23):15420-6. PMID: 1714457.
  • Expression of the gene coding for a human...