C80 Colorectal adenocarcinoma Cell Line

Colorectal cancer (CRC) cell lines are widely used pre-clinical model systems. Access the C80 colorectal adenocarcinoma cell line through CancerTools.org

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151759
Unit size: 1×10^6 cells / vial
Cancer types: Digestive / Gastrointestinal cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Rectum
Model: Tumour line
Cancer Types In Detail: Dukes' stage D;Human colorectal adenocarcinoma;Moderately well differentiated
Alternate name: C80; c80; C 80; C-80
Product description: The C80 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage D.
Conditional: Yes
Cellosaurus ID: CVCL_5249
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture routine: Cells grow as small dense islands and will not form a monolayer. C80 cells grow very slowly. Recommended that the cell line should be subcultured approximately every 5 days. On resuscitation, centrifugation of the cells (100g x 5 min) to remove DMSO improves the establishment of a viable culture. Seed cells at 2-4×10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA as a dissociation reagent. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells. Following resuscitation or subculture, the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Recommended growth conditions 5% CO ; 37°C.
References:

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.

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