C84 Colorectal adenocarcinoma Cell Line
Exclusive ToolThe C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes’ stage C.
Contributors
| Inventor | Institute |
|---|---|
| Walter Bodmer | University of Oxford |
| SKU: | 151760 |
|---|---|
| Product description: | The C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes’ stage C. |
| Conditional: | Yes |
| Cellosaurus ID: | CVCL_5250 |
| Cat. #: | 151760 |
|---|---|
| Provenance and Ownership: | The C84 Colorectal adenocarcinoma Cell Line is owned by Cancer Research UK. CancerTools supplies the C84 Colorectal adenocarcinoma Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing C84 Colorectal adenocarcinoma Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Colorectal cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Colon |
| Model: | Tumour line |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37?°C. Culture medium: Iscove’s Modified Dulbecco’s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine |
| References: |
Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898. Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
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