C84 Colorectal adenocarcinoma Cell Line

The C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes’ stage C.

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151760
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Product description: The C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes' stage C.
Conditional: Yes
Cellosaurus ID: CVCL_5250
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm?‹Â› using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37?‚°C. Culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
References:

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.

Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

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