C106 Colorectal Cell Line

The C106 cell line was established from a 78-year old female patient with a moderately well differentiated adenocarcinoma of the rectum

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151762
Unit size: 1×10^6 cells / vial
Cancer types: Adenocarcinoma
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Growth properties: Adherent
Cancer Types In Detail: Adenocarcinoma
Product description: The C106 cell line was established from a 78-year old female patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage A
Conditional: Yes
Cellosaurus ID: CVCL_M011
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine.
Subculture routine: Cells grow as small dense islands and will not form a monolayer. C106 cells grow very slowly. This cell line should be subcultured approximately every 5-8 days. On resuscitation, centrifugation of the cells (100g x 5 min) to remove DMSO improves the establishment of a viable culture. Seed cells at 4-7×10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells. Following resuscitation or subculture, the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Recommended growth conditions 5% CO2; 37°C. Please also see detailed protocol within the Product Datasheet in the Documentation section below.
Temperature: 37°C
Atmosphere: 5% CO2
References:

Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.

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