Cat. #151758
C75 Colorectal adenocarcinoma Cell Line
Cat. #: 151758
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Colon
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: University of Oxford, UK
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: C75 Colorectal adenocarcinoma Cell Line
- Alternate name: C75 Cell Line
- Cancer: Adenocarcinoma
- Cancers detailed: Adenocarcnoma of the sigmoid colon
- Research fields: Cancer;Drug development
- Organism: Human
- Tissue: Colon
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes' stage D.
- Cellosaurus id: CVCL_5248
Handling
- Format: Frozen
- Growth medium: Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37C. The cells grow very slowly - following resuscitation or subculture the cells take at least 48 hours
- Mycoplasma free: Yes
References
- Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.
