C75 Colorectal adenocarcinoma Cell Line

The C75 colorectal adenocarcinoma cell line was established from a 56-year old male patient. Access through CancerTools.org.

Contributors

Inventor Institute
Walter Bodmer University of Oxford, UK
Cat. #: 151758
Unit size: 1×10^6 cells / vial
Cancer types: Adenocarcinoma
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Cancer Types In Detail: Adenocarcnoma of the sigmoid colon
Alternate name: C75 Cell Line
Product description: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes' stage D.
Conditional: Yes
Cellosaurus ID: CVCL_5248
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture routine: Cells grow as small dense islands and will not form a monolayer. C75 cells grow very slowly. Recommended that the cell line should be subcultured approximately every 5 days. On resuscitation, centrifugation of the cells (100g x 5 min) to remove DMSO improves the establishment of a viable culture. Seed cells at 2-4×10,000 cells/cm² and use 0.05% trypsin or trypsin/EDTA as a dissociation reagent. Detached cells will often remain in clumps which may be further disaggregated by repeated pipetting of the cells. Following resuscitation or subculture, the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Recommended growth conditions 5% CO ; 37°C.
Temperature: 37° C
Atmosphere: 5% CO2
Mycoplasma free: Yes
References:

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.

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