#151758

C75 Colorectal adenocarcinoma Cell Line

Cat. #151758

C75 Colorectal adenocarcinoma Cell Line

Cat. #: 151758

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Walter Bodmer

Institute: University of Oxford, UK

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: C75 Colorectal adenocarcinoma Cell Line
  • Alternate name: C75 Cell Line
  • Cancer: Adenocarcinoma
  • Cancers detailed: Adenocarcnoma of the sigmoid colon
  • Research fields: Cancer;Drug development
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Description: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes' stage D.
  • Cellosaurus id: CVCL_5248

Handling

  • Format: Frozen
  • Growth medium: Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37C. The cells grow very slowly - following resuscitation or subculture the cells take at least 48 hours
  • Mycoplasma free: Yes

References

  • Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.