C70 Colorectal adenocarcinoma Cell Line

CRC cell lines are widely used pre-clinical model systems. The C70 colorectal adenocarcinoma cell line was established from a 60-year old female patient.

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151757
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Gender: Female
Model: Tumour line
Growth properties: Adherent
Product description: The C70 cell line was established from a 60-year old female patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes' stage B
Gender: Female
Conditional: No
Cellosaurus ID: CVCL_5247
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine.
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C70 cells grow slowly. Following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
References:

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-2845. PMID: 8464898.

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