C99 Colorectal adenocarcinoma Cell Line
Exclusive ToolA human colorectal adenocarcinoma cell line used to study mutation-driven signalling, therapeutic response, and precision oncology. Exclusive to CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.
Contributors
| Inventor | Institute |
|---|---|
| Walter Bodmer | University of Oxford |
| SKU: | 151761 |
|---|---|
| Product description: | The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes’ stage C |
| Conditional: | Yes |
| Cellosaurus ID: | CVCL_8176 |
| Disease: | Cancer |
| Cat. #: | 151761 |
|---|---|
| Provenance and Ownership: | The C99 Colorectal adenocarcinoma Cell Line is owned by Cancer Research UK. CancerTools supplies the C99 Colorectal adenocarcinoma Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing C99 Colorectal adenocarcinoma Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements. |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Colorectal cancer |
| Research Fields: | Cancer; Drug development |
| Organism: | Human |
| Tissue: | Colon |
| Model: | Tumour line |
| Cancer Types In Detail: | Adenocarcinoma |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | Iscove’s Modified Dulbecco’s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine |
| Subculture routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C99 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells. |
| References: |
Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639. Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. |
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C99 Colorectal adenocarcinoma Cell Line. 96 hours post plating. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)
