#151761

C99 Colorectal adenocarcinoma Cell Line

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Cat. #151761

C99 Colorectal adenocarcinoma Cell Line

Cat. #: 151761

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Tumour line

£575.00

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Contributor

Inventor: Walter Bodmer

Institute: University of Oxford

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: C99 Colorectal adenocarcinoma Cell Line
  • Cancer: Adenocarcinoma
  • Cancers detailed: Adenocarcinoma
  • Research fields: Cancer;Drug development
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Description: The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C
  • Cellosaurus id: CVCL_8176

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm?‹Â› using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37?‚° C. culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.
  • Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.