Cat. #151761
C99 Colorectal adenocarcinoma Cell Line
Cat. #: 151761
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Colon
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: C99 Colorectal adenocarcinoma Cell Line
- Cancer: Adenocarcinoma
- Cancers detailed: Adenocarcinoma
- Research fields: Cancer;Drug development
- Organism: Human
- Tissue: Colon
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C
- Cellosaurus id: CVCL_8176
Handling
- Format: Frozen
- Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C99 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
References
- Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.
- Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.
