C99 Colorectal adenocarcinoma Cell Line

C99 Colorectal adenocarcinoma Cell line established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum

Contributors

Inventor Institute
Walter Bodmer University of Oxford
Cat. #: 151761
Unit size: 1×10^6 cells / vial
Cancer types: Adenocarcinoma
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Cancer Types In Detail: Adenocarcinoma
Product description: The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C
Conditional: Yes
Cellosaurus ID: CVCL_8176
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C99 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
References:

Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.

Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.

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