Cat. #152845
BICR 3 Cell Line
Cat. #: 152845
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Alveolar ridge
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Eric Kenneth Parkinson
Institute: Cancer Research UK, Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BICR 3 Cell Line
- Alternate name: BICR-3; BICR3; Beatson Institute for Cancer Research 3
- Cancer type: Head and neck cancer
- Cancers detailed: Squamous cell carcinoma
- Research fields: Apoptosis and autophagy;Cancer;Drug development;Genetics
- Organism: Human
- Gender: Female
- Tissue: Alveolar ridge
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Receptors of note: Epidermal Growth Factor Receptor (EGFR)
- Description: Adherent keratinocyte cell line derived from a squamous cell carcinoma of the alveolus of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53 and p16. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.
- Additional notes: STR-PCR Data: Amelogenin: X, X CSF1PO: 11,12 D13S317: 8,13 D16S539: 11 D5S818: 11,12 D7S820: 10,13 THO1: 6,7 TPOX: 8 vWA: 14,19
- Cellosaurus id: CVCL_2311
Applications
- Application notes: STR-PCR Data: Amelogenin: X, X CSF1PO: 11,12 D13S317: 8,13 D16S539: 11 D5S818: 11,12 D7S820: 10,13 THO1: 6,7 TPOX: 8 vWA: 14,19
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:2 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density 4 x 10,000 cells/cm². A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line (however, this is not essential). Use of conditioned medium at a ratio of 1:1 with fresh medium can improve cell condition.
Related Tools
- Related tools: BICR 10 Cell Line ; BICR 16 Cell Line ; BICR 18 Cell Line ; BICR 22 Cell Line ; BICR 56 Cell Line ; BICR 6 Cell Line ; BICR 78 Cell Line ; BICR 31 Cell Line ; BICR 82 Cell Line
References
- Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.
