A549 CisR cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: A549 CisR cell line
• Alternate name: A549 Chemo-resistant cell line
• Parental cell: A549
• Organism: Human
• Gender: Male
• Tissue: Lung
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Therapy Resistance / Drug (Cisplatin) / Extrinsic
• Description: The developmen of acquired cisplatin resistance is a significant clinical challenge in the treatment of NSCLC patients. An isogenic panel of cisplatin resistant (CisR) A549 cell lines were generated from their wild-type/parental (PT) counterparts and extensively characterised. These provide a valuable in vitro tool for investigating molecular pathways and mechanisms of resistance that may be associated with the cisplatin resistant phenotype in lung cancer patients.

Handling

• Format: Frozen
• Passage number: A549 CisR (P85)
• Growth medium: Hams-F12 media supplemented with 10% FBS, 1% Penicillin/Streptomycin, 4mM L-Glutamine (final concentration)
• Atmosphere: 5% CO2
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Subculture routine: Subculture cells using trypsin-EDTA. Neutralise trypsin in supplemented cell culture media and pellet cells by centrifugation at 1300 rpm for 3 min. Discard supernatant to waste and resuspend the cell pellet in 1 mL of supplmented cell culture media. Transfer cell content to a T75 cm2 vented culture flask and incubate until further use.
• Cultured in antibiotics: Penicillin, Streptomycin
• Characterisation tests: RT-PCR
• Str profiling: Parent (PT) cells profiled in 2014 - confirmed authentication

References

• Barr MP, et al. 2013. PLoS One. 8(1):e54193. PMID: 23349824