#152706

A2780 Cell Line

Cat. #152706

A2780 Cell Line

Cat. #: 152706

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Ovary

Disease: Cancer

Model: Tumour line

£800.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Military Institute of Medicine - National Research Institute

Tool Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: A2780 Cell Line
  • Organism: Human
  • Tissue: Ovary
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: A2780 cell line used to investigate cisplatin resistance in ovarian cancer. Background and Research Application The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient of ovarian endometroid adenocarcinoma. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (catalogue no. 152708) and the adriamycin resistant cell line A2780 ADR (catalogue no. 152707). This cell line has been used in a variety of research, including understanding the role of Snail and Slug in ovarian tumour neovascularization; how to overcome cisplatin resistance in ovarian cancer cells using liposome application and studying the effect of low molecular weight heparin tinzaparin on cisplatin resistance in ovarian cancer cells.
  • Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
  • Production details: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
  • Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16
  • Cellosaurus id: CVCL_0134

Applications

  • Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
  • Application notes: Points of Interest STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16 Concentration Vial has between 1-5 million cells as standard, however this may vary.

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

Related Tools

  • Related tools: A2780ADR Cell Line ; A2780cis Cell Line

References

  • Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.
  • Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.
  • Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.
  • Zhao et al. 2017. Clin Proteomics. 14:20. PMID: 28546799.
  • Medrano et al. 2017. Cell Rep. 18(10):2343-2358. PMID: 28273451.
  • Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line.
  • Januchowski et al. 2014. Biomed Res Int. 2014:365867. Parker et al. 1991. J Clin Invest. 87(3):772-7. PMID: 1999494.